Creatine Kinase, Brain (CKB) antibody

Antigen: Creatine Kinase, Brain (CKB)

Synonyms: B-CK, CKBB, Bck, Ck3, Ck-3, Ckbb, bck, ckb, cb594, wu:fj36f10, wu:fj37d11, MGC53426, CKB, b-ck, ckbb, Ckbr, RATCKBR

Clonality: Monoclonal (3D5-3D11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393326

Additional Information

Alternative name: CKB

Gene ID: 1152

Immunogen: CKB (AAH01190, 1 a.a. ~ 382 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3D5-3D11

Description: Other names: BCK, CKBBcreatine kinase, brain

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CKB (AAH01190, 1 a.a. ~ 382 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 42644 DA

Comments:

Background: The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 1mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to CKB (AT1543a) on HeLa cell . [antibody concentration 30 ug/ml]

Publications

Product: Martins-de-Souza, Gattaz, Schmitt et al.: "Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia." in: European archives of psychiatry and clinical neuroscience, Vol. 259, Issue 3, pp. 151-63, 2009 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation." in: BMC psychiatry, Vol. 9, pp. 17, 2009 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Kim, Amante, Moody et al.: "Reduced creatine kinase as a central and peripheral biomarker in Huntington's disease." in: Biochimica et biophysica acta, Vol. 1802, Issue 7-8, pp. 673-81, 2010 (PubMed).

Steen, Wilczak, Hoogduin et al.: "Reduced creatine kinase B activity in multiple sclerosis normal appearing white matter." in: PLoS ONE, Vol. 5, Issue 5, pp. e10811, 2010 (PubMed).