ADAM Metallopeptidase Domain 2 (ADAM2) antibody

Antigen: ADAM Metallopeptidase Domain 2 (ADAM2)

Synonyms: CT15, FTNB, PH30, CRYN1, CRYN2, PH-30b

Clonality: Monoclonal (4A2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393338

Additional Information

Alternative name: ADAM2

Gene ID: 2515

UniProt: NP_001455

Immunogen: ADAM2 (NP_001455, 376 a.a. ~ 476 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4A2

Description: Other names: FTNB, PH30b, PH30 a disintegrin and metalloproteinase domain 2 (fertilin beta)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ADAM2 (NP_001455, 376 a.a. ~ 476 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 82457 DA

Comments:

Background: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a subunit of an integral sperm membrane glycoprotein called fertilin, which plays an important role in sperm-egg interactions.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Neurology

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged ADAM2 is approximately 0.1ng/ml as a capture antibody.

Publications

Product: Ikawa, Nakanishi, Yamada et al.: "Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility." in: Developmental biology, Vol. 240, Issue 1, pp. 254-61, 2002 (PubMed).

Eto, Huet, Tarui et al.: "Functional classification of ADAMs based on a conserved motif for binding to integrin alpha 9beta 1: implications for sperm-egg binding and other cell interactions." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 17804-10, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Depa-Martynów, Kempisty, Lianeri et al.: "Association between fertilin beta, protamines 1 and 2 and spermatid-specific linker histone H1-like protein mRNA levels, fertilization ability of human spermatozoa, and quality of preimplantation embryos." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45 Suppl 1, pp. S79-85, 2008 (PubMed).