|+1 404 474 4654|
|+1 888 205 9894 (TF)|
ADAM Metallopeptidase Domain 2 (ADAM2) antibody
|Synonyms||CT15, FTNB, PH30, CRYN1, CRYN2, PH-30b|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||ADAM2 (NP_001455, 376 a.a. ~ 476 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: FTNB, PH30b, PH30 a disintegrin and metalloproteinase domain 2 (fertilin beta)|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||ADAM2 (NP_001455, 376 a.a. ~ 476 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||82457 DA|
Background: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a subunit of an integral sperm membrane glycoprotein called fertilin, which plays an important role in sperm-egg interactions.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Signaling, Cell Structure, Neurology|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged ADAM2 is approximately 0.1ng/ml as a capture antibody.|
Ikawa, Nakanishi, Yamada et al.: "Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility." in: Developmental biology, Vol. 240, Issue 1, pp. 254-61, 2002 (PubMed).
Eto, Huet, Tarui et al.: "Functional classification of ADAMs based on a conserved motif for binding to integrin alpha 9beta 1: implications for sperm-egg binding and other cell interactions." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 17804-10, 2002 (PubMed).
Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).
Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).
Depa-Martynów, Kempisty, Lianeri et al.: "Association between fertilin beta, protamines 1 and 2 and spermatid-specific linker histone H1-like protein mRNA levels, fertilization ability of human spermatozoa, and quality of preimplantation embryos." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45 Suppl 1, pp. S79-85, 2008 (PubMed).
|Hosts||Rabbit (24), Mouse (2)|
|Reactivities||Human (24), Mouse (Murine) (1)|
|Applications||Immunofluorescence (IF) (16), Western Blotting (WB) (11), ELISA (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (2), Internal Region (1)|