CES2 antibody

Antigen: CES2

Clonality: Monoclonal (4F12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393347

Additional Information

Gene ID: 8824

UniProt: NP_003860

Immunogen: CES2 (NP_003860, 514 a.a. ~ 622 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 4F12

Description: Other names: CE-2, iCE carboxylesterase 2 (intestine, liver)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CES2 (NP_003860, 514 a.a. ~ 622 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 61807 DA

Comments:

Background: This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

CES2 monoclonal antibody (M02), clone 4F12 Western Blot analysis of CES2 expression in HepG2 ( Cat # L019V1 ). Immunoperoxidase of monoclonal antibody to CES2 ( Cat # AT1499a ) on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

anti-CES2 antibody (Image 2)

Publications

Product: Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Holmes, Cox, VandeBerg: "Mammalian carboxylesterase 3: comparative genomics and proteomics." in: Genetica, Vol. 138, Issue 7, pp. 695-708, 2010 (PubMed).

Howard, Hsu, Grzywacz et al.: "Evaluation of candidate genes for cholinesterase activity in farmworkers exposed to organophosphorus pesticides: association of single nucleotide polymorphisms in BCHE." in: Environmental health perspectives, Vol. 118, Issue 10, pp. 1395-9, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).