Protocadherin 1 (PCDH1) antibody

Antigen: Protocadherin 1 (PCDH1)

Synonyms: PC42, PCDH42, FLJ53887, MGC45991

Clonality: Monoclonal (5D5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393359

Additional Information

Alternative name: PCDH1

Gene ID: 5097

UniProt: NP_002578

Immunogen: PCDH1 (NP_002578, 62 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 5D5

Description: Other names: MGC45991, PC42, PCDH42 protocadherin 1 (cadherin-like 1)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: PCDH1 (NP_002578, 62 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 111270 DA

Comments:

Background: This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Neurology

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to PCDH1 (AT3210a) on A-431 cell . [antibody concentration 10 ug/ml] PCDH1 monoclonal antibody (M01), clone 5D5 Western Blot analysis of PCDH1 expression in A-431 ( Cat # L015V1 ).

anti-Protocadherin 1 (PCDH1) antibody (Image 2)

Publications

Product: Colland, Jacq, Trouplin et al.: "Functional proteomics mapping of a human signaling pathway." in: Genome research, Vol. 14, Issue 7, pp. 1324-32, 2004 (PubMed).

Ballif, Villén, Beausoleil et al.: "Phosphoproteomic analysis of the developing mouse brain." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 11, pp. 1093-101, 2004 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Otsuki, Ota, Nishikawa et al.: "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 12, Issue 2, pp. 117-26, 2005 (PubMed).

Koppelman, Meyers, Howard et al.: "Identification of PCDH1 as a novel susceptibility gene for bronchial hyperresponsiveness." in: American journal of respiratory and critical care medicine, Vol. 180, Issue 10, pp. 929-35, 2009 (PubMed).