Protocadherin gamma Subfamily A, 5 (PCDHGA5) antibody

Antigen: Protocadherin gamma Subfamily A, 5 (PCDHGA5)

Synonyms: ME3, MGC142020, CDH-GAMMA-A5, PCDH-GAMMA-A5

Clonality: Monoclonal (5H5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393365

Additional Information

Alternative name: PCDHGA5

Gene ID: 56110

UniProt: NP_061741

Immunogen: PCDHGA5 (NP_061741, 205 a.a. ~ 305 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 5H5

Description: Other names: CDHGAMMAA5, ME3, PCDHGAMMAA5 protocadherin gamma subfamily A, 5

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: PCDHGA5 (NP_061741, 205 a.a. ~ 305 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 100935 DA

Comments:

Background: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western Blot analysis of PCDHGA5 expression in transfected 293T cell line by PCDHGA5 monoclonal antibody (M01), clone 5H5. Lane 1: PCDHGA5 transfected lysate(100.935 KDa). Lane 2: Non-transfected lysate. Detection limit for recombinant GST tagged PCDHGA5 is approximately 0.1ng/ml as a capture antibody.

anti-Protocadherin gamma Subfamily A, 5 (PCDHGA5) antibody (Image 2)

Publications

Product: Wu, Maniatis: "Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3124-9, 2000 (PubMed).

Yagi, Takeichi: "Cadherin superfamily genes: functions, genomic organization, and neurologic diversity." in: Genes & development, Vol. 14, Issue 10, pp. 1169-80, 2000 (PubMed).

Nollet, Kools, van Roy: "Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members." in: Journal of molecular biology, Vol. 299, Issue 3, pp. 551-72, 2000 (PubMed).

Wu, Zhang, Cheng et al.: "Comparative DNA sequence analysis of mouse and human protocadherin gene clusters." in: Genome research, Vol. 11, Issue 3, pp. 389-404, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).