Cadherin 18, Type 2 (CDH18) antibody

Antigen: Cadherin 18, Type 2 (CDH18)

Synonyms: CDH14, CDH24, CDH14L, EY-CADHERIN

Clonality: Monoclonal (6F7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393369

Additional Information

Alternative name: CDH18

Gene ID: 1016

UniProt: NP_004925

Immunogen: CDH18 (NP_004925, 467 a.a. ~ 577 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 6F7

Description: Other names: CDH14, CDH14L, CDH24, EYCADHERIN cadherin 18, type 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CDH18 (NP_004925, 467 a.a. ~ 577 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 88073 DA

Comments:

Background: This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed specifically in the central nervous system and is putatively involved in synaptic adhesion, axon outgrowth and guidance.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Neurology

Restrictions: For Research Use only

Images

CDH18 monoclonal antibody (M01), clone 6F7 Western Blot analysis of CDH18 expression in HeLa ( Cat # L013V1 ). Immunoperoxidase of monoclonal antibody to CDH18 ( Cat # AT1473a ) on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

anti-Cadherin 18, Type 2 (CDH18) antibody (Image 2)

Publications

Product: Shimoyama, Tsujimoto, Kitajima et al.: "Identification of three human type-II classic cadherins and frequent heterophilic interactions between different subclasses of type-II classic cadherins." in: The Biochemical journal, Vol. 349, Issue Pt 1, pp. 159-67, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Yang, Yuan, Gao et al.: "[Expressions of cadherin molecules CDH18 and PCDH17 in human azoospermic testes]." in: Zhonghua nan ke xue = National journal of andrology, Vol. 15, Issue 12, pp. 1081-4, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).