Minichromosome Maintenance Complex Component 3 (MCM3) (AA 699-809) antibody

Details for Product No. ABIN393375
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Antigen
Synonyms CG4206, DmMCM3, DmMcm3, DmeMCM3, Dmel\\CG4206, MCM3, McM3, Mcp-PCR2, PCR2, dMCM3, cb32, chunp6867, wu:fa26g03, HCC5, P1-MCM3, P1.h, RLFB, xmcm3, AL033361, C80350, Mcmd, P1, p1.m
Epitope
AA 699-809
(9), (8), (7), (6), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(72), (25), (22), (14), (13), (12), (1), (1), (1)
Host
Mouse
(49), (17), (9)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(64), (28), (15), (13), (11), (10), (9), (5), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393375
450.00 $
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Immunogen MCM3 (NP_002379, 699 a.a. ~ 809 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3E1
Isotype IgG2b kappa
Specificity MCM3 (NP_002379, 699 a.a. ~ 809 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MCM3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 90981 DA
Gene ID 12406
NCBI Accession NP_002379
Research Area Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Minichromosome Maintenance Complex Component 3 (MCM3) (AA 699-809) antibody Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell (antibody concentration 10 µg/mL). MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa.
anti-Minichromosome Maintenance Complex Component 3 (MCM3) (AA 699-809) antibody (2) anti-Minichromosome Maintenance Complex Component 3 (MCM3) (AA 699-809) antibody (Image 2)
Background publications Lin, Aggarwal, Diehl: "Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 8079-84, 2008 (PubMed).

Lee, Ha, Kim et al.: "Minichromosome maintenance protein 3 is a candidate proliferation marker in papillary thyroid carcinoma." in: Experimental and molecular pathology, Vol. 88, Issue 1, pp. 138-42, 2010 (PubMed).

Saade, Mechold, Kulyyassov et al.: "Analysis of interaction partners of H4 histone by a new proteomics approach." in: Proteomics, Vol. 9, Issue 21, pp. 4934-43, 2009 (PubMed).

Olson, Wang, Pankratz et al.: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010 (PubMed).

Song, Stinski: "Human cytomegalovirus IE86 protein binds to cellular Mcm3 protein but does not inhibit its binding to the Epstein-Barr virus oriP in U373MG-p220.2 cells." in: Acta virologica, Vol. 54, Issue 2, pp. 125-30, 2010 (PubMed).

Validation Images
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