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Minichromosome Maintenance Complex Component 3 (MCM3) (AA 699-809) antibody

Details for Product No. ABIN393375, Supplier: Log in to see
Antigen
  • CG4206
  • DmMCM3
  • DmMcm3
  • DmeMCM3
  • Dmel\\CG4206
  • MCM3
  • McM3
  • Mcp-PCR2
  • PCR2
  • dMCM3
  • cb32
  • chunp6867
  • wu:fa26g03
  • HCC5
  • P1-MCM3
  • P1.h
  • RLFB
  • xmcm3
  • AL033361
  • C80350
  • Mcmd
  • P1
  • p1.m
Alternatives
anti-Human Minichromosome Maintenance Complex Component 3 antibody for Western Blotting
Epitope
AA 699-809
22
15
7
6
6
4
3
3
1
1
1
1
1
1
1
1
1
Reactivity
Human
90
33
30
2
1
1
Host
Mouse
73
13
5
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
73
40
17
16
15
11
9
3
2
1
1
1
Supplier
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Immunogen MCM3 (NP_002379, 699 a.a. ~ 809 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3E1
Isotype IgG2b kappa
Specificity MCM3 (NP_002379, 699 a.a. ~ 809 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MCM3 (MCM3 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 90981 DA
Gene ID 12406
NCBI Accession NP_002379
Research Area Signaling, Protein Modifications, Chromatin, Cell Structure
Pathways DNA Damage Repair
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Minichromosome Maintenance Complex Component 3 (MCM3) (AA 699-809) antibody (ABIN393375) Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-Minichromosome Maintenance Complex Component 3 (MCM3) (AA 699-809) antibody (ABIN393375) anti-Minichromosome Maintenance Complex Component 3 (MCM3) (AA 699-809) antibody (Image 2)
Background publications Song, Stinski: "Human cytomegalovirus IE86 protein binds to cellular Mcm3 protein but does not inhibit its binding to the Epstein-Barr virus oriP in U373MG-p220.2 cells." in: Acta virologica, Vol. 54, Issue 2, pp. 125-30, 2010 (PubMed).

Olson, Wang, Pankratz et al.: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010 (PubMed).

Saade, Mechold, Kulyyassov et al.: "Analysis of interaction partners of H4 histone by a new proteomics approach." in: Proteomics, Vol. 9, Issue 21, pp. 4934-43, 2009 (PubMed).

Lee, Ha, Kim et al.: "Minichromosome maintenance protein 3 is a candidate proliferation marker in papillary thyroid carcinoma." in: Experimental and molecular pathology, Vol. 88, Issue 1, pp. 138-42, 2010 (PubMed).

Lin, Aggarwal, Diehl: "Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 8079-84, 2008 (PubMed).