Minichromosome Maintenance Complex Component 3 (MCM3) antibody

Antigen: Minichromosome Maintenance Complex Component 3 (MCM3)

Synonyms: HCC5, P1.h, RLFB, MGC1157, P1-MCM3, mcm3, MGC53530

Clonality: Monoclonal (3E1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393375

Additional Information

Alternative name: MCM3

Gene ID: 4172

UniProt: NP_002379

Immunogen: MCM3 (NP_002379, 699 a.a. ~ 809 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3E1

Description: Other names: HCC5, MGC1157, P1MCM3, P1.h, RLFB MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MCM3 (NP_002379, 699 a.a. ~ 809 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 90981 DA

Comments:

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to MCM3 (AT2820a) on HeLa cell . [antibody concentration 10 ug/ml] MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa ( Cat # L013V1 ).

anti-Minichromosome Maintenance Complex Component 3 (MCM3) antibody (Image 2)

Publications

Product: Lin, Aggarwal, Diehl: "Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 8079-84, 2008 (PubMed).

Lee, Ha, Kim et al.: "Minichromosome maintenance protein 3 is a candidate proliferation marker in papillary thyroid carcinoma." in: Experimental and molecular pathology, Vol. 88, Issue 1, pp. 138-42, 2010 (PubMed).

Saade, Mechold, Kulyyassov et al.: "Analysis of interaction partners of H4 histone by a new proteomics approach." in: Proteomics, Vol. 9, Issue 21, pp. 4934-43, 2009 (PubMed).

Olson, Wang, Pankratz et al.: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010 (PubMed).

Song, Stinski: "Human cytomegalovirus IE86 protein binds to cellular Mcm3 protein but does not inhibit its binding to the Epstein-Barr virus oriP in U373MG-p220.2 cells." in: Acta virologica, Vol. 54, Issue 2, pp. 125-30, 2010 (PubMed).