|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Minichromosome Maintenance Complex Component 3 (MCM3) antibody
|Synonyms||HCC5, P1.h, RLFB, MGC1157, P1-MCM3, mcm3, MGC53530|
Alternatives ELISA, Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||MCM3 (NP_002379, 699 a.a. ~ 809 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: HCC5, MGC1157, P1MCM3, P1.h, RLFB MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||MCM3 (NP_002379, 699 a.a. ~ 809 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||90981 DA|
Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Lin, Aggarwal, Diehl: "Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 8079-84, 2008 (PubMed).
Lee, Ha, Kim et al.: "Minichromosome maintenance protein 3 is a candidate proliferation marker in papillary thyroid carcinoma." in: Experimental and molecular pathology, Vol. 88, Issue 1, pp. 138-42, 2010 (PubMed).
Saade, Mechold, Kulyyassov et al.: "Analysis of interaction partners of H4 histone by a new proteomics approach." in: Proteomics, Vol. 9, Issue 21, pp. 4934-43, 2009 (PubMed).
Olson, Wang, Pankratz et al.: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010 (PubMed).
Song, Stinski: "Human cytomegalovirus IE86 protein binds to cellular Mcm3 protein but does not inhibit its binding to the Epstein-Barr virus oriP in U373MG-p220.2 cells." in: Acta virologica, Vol. 54, Issue 2, pp. 125-30, 2010 (PubMed).
|Hosts||Rabbit (35), Goat (5), Mouse (2)|
|Reactivities||Human (39), Mouse (Murine) (26), Rat (Rattus) (25), Cow (Bovine) (21), Dog (Canine) (20), Horse (Equine) (18), Pig (Porcine) (18), Chicken (2), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (27), Immunofluorescence (IF) (17), ELISA (15), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (2), AA 692-708 (1), N-Term (1)|