Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2)

Synonyms: UBC7, Ubc7p, D10Xrf369, 1110003O05Rik, wu:fu51c05, wu:fu56g11, UBE2G2, MGC140569, DKFZp469F0921, MGC82328, ubc7

Clonality: Monoclonal (5E1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393378

Additional Information

Alternative name: UBE2G2

Gene ID: 7327

UniProt: NP_003334

Immunogen: UBE2G2 (-, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 5E1

Description: Other names: UBC7ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: UBE2G2 (-, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 18566 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Concentration: 0.26mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

UBE2G2 monoclonal antibody (M01), clone 5E1 Western Blot analysis of UBE2G2 expression in HeLa ( Cat # L013V1 ). Immunoperoxidase of monoclonal antibody to UBE2G2 ( Cat # AT4436a ) on formalin-fixed paraffin-embedded human liver. [antibody concentration 1 ug/ml]

anti-Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) antibody (Image 2)

Publications

Product: Hu, Warnatz, Vanhecke et al.: "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins." in: BMC genomics, Vol. 7, pp. 155, 2006 (PubMed).

Li, Tu, Brunger et al.: "A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate." in: Nature, Vol. 446, Issue 7133, pp. 333-7, 2007 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Cao, Wang, Qi et al.: "Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism by regulating the stability of HMG-CoA reductase." in: Cell metabolism, Vol. 6, Issue 2, pp. 115-28, 2007 (PubMed).

Ju, Bocik, Majumdar et al.: "Solution structure and dynamics of human ubiquitin conjugating enzyme Ube2g2." in: Proteins, Vol. 78, Issue 5, pp. 1291-301, 2010 (PubMed).