Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) antibody

Details for Product No. ABIN393378
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Synonyms UBC7, Ubc7p, D10Xrf369, 1110003O05Rik, wu:fu51c05, wu:fu56g11, zgc:112077, ubc7
(32), (15), (14), (12), (12), (12)
(23), (9)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(21), (11), (11), (10), (7), (2), (1), (1)
Pubmed 5 references available
Catalog no. ABIN393378
Quantity 0.1 mg
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Immunogen UBE2G2 (-, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5E1
Isotype IgG2a kappa
Specificity UBE2G2 (-, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2G2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 18566 DA
Gene ID 12406
UniProt NP_003334
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Restrictions For Research Use only
Concentration 0.26mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) antibody UBE2G2 monoclonal antibody (M01), clone 5E1 Western Blot analysis of UBE2G2 expression in HeLa on formalin-fixed paraffin-embedded human liver (antibody concentration 1 µg/mL).
anti-Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) antibody (2) anti-Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) antibody (Image 2)
Background publications Hu, Warnatz, Vanhecke et al.: "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins." in: BMC genomics, Vol. 7, pp. 155, 2006 (PubMed).

Li, Tu, Brunger et al.: "A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate." in: Nature, Vol. 446, Issue 7133, pp. 333-7, 2007 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Cao, Wang, Qi et al.: "Ufd1 is a cofactor of gp78 and plays a key role in cholesterol metabolism by regulating the stability of HMG-CoA reductase." in: Cell metabolism, Vol. 6, Issue 2, pp. 115-28, 2007 (PubMed).

Ju, Bocik, Majumdar et al.: "Solution structure and dynamics of human ubiquitin conjugating enzyme Ube2g2." in: Proteins, Vol. 78, Issue 5, pp. 1291-301, 2010 (PubMed).

Hosts (23), (9)
Reactivities (32), (15), (14), (12), (12), (12)
Applications (21), (11), (11), (10), (7), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1), (1), (1)
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