Asparagine-Linked Glycosylation 12, alpha-1,6-Mannosyltransferase Homolog (S. Cerevisiae) (ALG12) (AA 369-426) antibody

Details for Product No. ABIN393387
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Antigen
Synonyms si:dkey-164m14.3, CDG1G, ECM39, PP14673, hALG12
Epitope
AA 369-426
(2), (1)
Reactivity
Human
(3), (1)
Host
Mouse
(2), (1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393387
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Immunogen ALG12 (NP_077010, 369 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5E3
Isotype IgG1 kappa
Specificity ALG12 (NP_077010, 369 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ALG12
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 54655 DA
Gene ID 12406
NCBI Accession NP_077010
Research Area Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Asparagine-Linked Glycosylation 12, alpha-1,6-Mannosyltransferase Homolog (S. Cerevisiae) (ALG12) (AA 369-426) antibody ALG12 monoclonal antibody (M06), clone 5E3 Western Blot analysis of ALG12 expression in HeLa. Detection limit for recombinant GST tagged ALG12 is approximately 0.3 ng/mL as a capture antibody.
anti-Asparagine-Linked Glycosylation 12, alpha-1,6-Mannosyltransferase Homolog (S. Cerevisiae) (ALG12) (AA 369-426) antibody (2) anti-Asparagine-Linked Glycosylation 12, alpha-1,6-Mannosyltransferase Homolog (S. Cerevisiae) (ALG12) (AA 369-426) antibody (Image 2)
Background publications Jaeken: "Congenital disorders of glycosylation (CDG): update and new developments." in: Journal of inherited metabolic disease, Vol. 27, Issue 3, pp. 423-6, 2004 (PubMed).

Jaeken, Carchon: "Congenital disorders of glycosylation: a booming chapter of pediatrics." in: Current opinion in pediatrics, Vol. 16, Issue 4, pp. 434-9, 2004 (PubMed).

Collins, Wright, Edwards et al.: "A genome annotation-driven approach to cloning the human ORFeome." in: Genome biology, Vol. 5, Issue 10, pp. R84, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Wan, Gong, Qin et al.: "Large-scale cDNA transfection screening for genes related to cancer development and progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 44, pp. 15724-9, 2004 (PubMed).

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