Asparagine-Linked Glycosylation 12, alpha-1,6-Mannosyltransferase Homolog (S. Cerevisiae) (ALG12) antibody

Antigen: Asparagine-Linked Glycosylation 12, alpha-1,6-Mannosyltransferase Homolog (S. Cerevisiae) (ALG12)

Synonyms: ECM39, hALG12, MGC3136, PP14673, MGC111358, MGC29374, ALG12, si:dkey-164m14.3

Clonality: Monoclonal (5E3)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393387

Additional Information

Alternative name: ALG12

Gene ID: 79087

UniProt: NP_077010

Immunogen: ALG12 (NP_077010, 369 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 5E3

Description: Other names: ECM39, MGC111358, MGC3136, PP14673, hALG12asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ALG12 (NP_077010, 369 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 54655 DA

Comments:

Background: This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

ALG12 monoclonal antibody (M06), clone 5E3 Western Blot analysis of ALG12 expression in HeLa ( Cat # L013V1 ). Detection limit for recombinant GST tagged ALG12 is approximately 0.3ng/ml as a capture antibody.

anti-Asparagine-Linked Glycosylation 12, alpha-1,6-Mannosyltransferase Homolog (S. Cerevisiae) (ALG12) antibody (Image 2)

Publications

Product: Jaeken: "Congenital disorders of glycosylation (CDG): update and new developments." in: Journal of inherited metabolic disease, Vol. 27, Issue 3, pp. 423-6, 2004 (PubMed).

Jaeken, Carchon: "Congenital disorders of glycosylation: a booming chapter of pediatrics." in: Current opinion in pediatrics, Vol. 16, Issue 4, pp. 434-9, 2004 (PubMed).

Collins, Wright, Edwards et al.: "A genome annotation-driven approach to cloning the human ORFeome." in: Genome biology, Vol. 5, Issue 10, pp. R84, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Wan, Gong, Qin et al.: "Large-scale cDNA transfection screening for genes related to cancer development and progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 44, pp. 15724-9, 2004 (PubMed).