ADP-Dependent Glucokinase (ADPGK) antibody

Details for Product No. ABIN393388
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Synonyms 2610017G09Rik, ADP-GK, Adp-gk, C77577, RGD1306103, ADPGK
(28), (19), (17), (4), (3)
(25), (3)
Clonality (Clone)
Monoclonal ()
Western Blotting (WB)
(28), (13), (5), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393388
Quantity 0.1 mg
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Immunogen ADPGK (NP_112574, 425 a.a. ~ 497 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E4
Isotype IgG2a kappa
Specificity ADPGK (NP_112574, 425 a.a. ~ 497 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ADPGK
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 54089 DA
Gene ID 12406
UniProt NP_112574
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000

Background: ADPGK (EC catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-ADP-Dependent Glucokinase (ADPGK) antibody Western Blot analysis of ADPGK expression in transfected 293T cell line by ADPGK monoclonal antibody (M01), clone 1E4. Lane 1: ADPGK transfected lysate(54.1 KDa). Lane 2: Non-transfected lysate. Western blot analysis of ADPGK over-expressed 293 cell line, cotransfected with ADPGK Validated Chimera RNAi used as specificity and loading control.
anti-ADP-Dependent Glucokinase (ADPGK) antibody (2) anti-ADP-Dependent Glucokinase (ADPGK) antibody (Image 2)
Background publications Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Codner, Rocha, Deng et al.: "Mild fasting hyperglycemia in children: high rate of glucokinase mutations and some risk of developing type 1 diabetes mellitus." in: Pediatric diabetes, Vol. 10, Issue 6, pp. 382-8, 2009 (PubMed).

Cuesta-Muñoz, Tuomi, Cobo-Vuilleumier et al.: "Clinical heterogeneity in monogenic diabetes caused by mutations in the glucokinase gene (GCK-MODY)." in: Diabetes Care, Vol. 33, Issue 2, pp. 290-2, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Hosts (25), (3)
Reactivities (28), (19), (17), (4), (3)
Applications (28), (13), (5), (2), (1)
Epitopes (5), (3), (2), (2), (1), (1)
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