ADP-Dependent Glucokinase (ADPGK) antibody

Antigen: ADP-Dependent Glucokinase (ADPGK)

Synonyms: ADP-GK, DKFZp434B195, 2610017G09Rik, Adp-gk, C77577, RGD1306103, ADPGK, MGC148410

Clonality: Monoclonal (1E4)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393388

Additional Information

Alternative name: ADPGK

Gene ID: 83440

UniProt: NP_112574

Immunogen: ADPGK (NP_112574, 425 a.a. ~ 497 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1E4

Description: Other names: 2610017G09Rik, ADPGK, DKFZP434B195 ADP-dependent glucokinase

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ADPGK (NP_112574, 425 a.a. ~ 497 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 54089 DA

Comments:

Background: ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of ADPGK expression in transfected 293T cell line by ADPGK monoclonal antibody (M01), clone 1E4. Lane 1: ADPGK transfected lysate(54.1 KDa). Lane 2: Non-transfected lysate. Western blot analysis of ADPGK over-expressed 293 cell line, cotransfected with ADPGK Validated Chimera RNAi ( Cat # H00083440-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ADPGK monoclonal antibody (M01), clone 1E4 (Cat # AT1057a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-ADP-Dependent Glucokinase (ADPGK) antibody (Image 2)

Publications

Product: Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Codner, Rocha, Deng et al.: "Mild fasting hyperglycemia in children: high rate of glucokinase mutations and some risk of developing type 1 diabetes mellitus." in: Pediatric diabetes, Vol. 10, Issue 6, pp. 382-8, 2009 (PubMed).

Cuesta-Muñoz, Tuomi, Cobo-Vuilleumier et al.: "Clinical heterogeneity in monogenic diabetes caused by mutations in the glucokinase gene (GCK-MODY)." in: Diabetes Care, Vol. 33, Issue 2, pp. 290-2, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).