Male Germ Cell-Associated Kinase (MAK) antibody

Antigen: Male Germ Cell-Associated Kinase (MAK)

Synonyms: dJ417M14.2, A930010O05Rik, MGC93585, Ick, fj04c02, zgc:56603, wu:fj04c02, xmak, MGC82717, MGC134523, MGC146434

Clonality: Monoclonal (3E5)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393396

Additional Information

Alternative name: MAK

Gene ID: 4117

Immunogen: MAK (AAH39825, 358 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3E5

Description: Other names: dJ417M14.2 male germ cell-associated kinase

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MAK (AAH39825, 358 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 70581 DA

Comments:

Background: The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to MAK (AT2767a) on HepG2 cell . [antibody concentration 10 ug/ml] MAK monoclonal antibody (M01), clone 3E5 Western Blot analysis of MAK expression in HepG2 ( Cat # L019V1 ).

anti-Male Germ Cell-Associated Kinase (MAK) antibody (Image 2)

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Mungall, Palmer, Sims et al.: "The DNA sequence and analysis of human chromosome 6." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Ma, Xia, Desai et al.: "Male germ cell-associated kinase, a male-specific kinase regulated by androgen, is a coactivator of androgen receptor in prostate cancer cells." in: Cancer research, Vol. 66, Issue 17, pp. 8439-47, 2006 (PubMed).

Fu, Larson, Chitta et al.: "Identification of yin-yang regulators and a phosphorylation consensus for male germ cell-associated kinase (MAK)-related kinase." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8639-54, 2006 (PubMed).