Arylacetamide Deacetylase (Esterase) (AADAC) antibody

Antigen: Arylacetamide Deacetylase (Esterase) (AADAC)

Synonyms: Aada, AI265437, 5033417E09Rik, MGC89272, AADAC, DAC, CES5A1, MGC142384

Clonality: Monoclonal (2E8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393404

Additional Information

Alternative name: AADAC

Gene ID: 13

UniProt: NP_001077

Immunogen: AADAC (NP_001077, 201 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2E8

Description: Other names: DAC arylacetamide deacetylase (esterase)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AADAC (NP_001077, 201 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 45748 DA

Comments:

Background: Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged AADAC is approximately 3ng/ml as a capture antibody.

Publications

Product: Ross, Crow: "Human carboxylesterases and their role in xenobiotic and endobiotic metabolism." in: Journal of biochemical and molecular toxicology, Vol. 21, Issue 4, pp. 187-96, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Watanabe, Fukami, Nakajima et al.: "Human arylacetamide deacetylase is a principal enzyme in flutamide hydrolysis." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 37, Issue 7, pp. 1513-20, 2009 (PubMed).

Saito, Kawamoto, Kamatani: "Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects." in: Journal of human genetics, Vol. 54, Issue 6, pp. 317-23, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).