Male Germ Cell-Associated Kinase (MAK) antibody

Details for Product No. ABIN393408
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms Ick, fj04c02, zgc:56603, wu:fj04c02, RP62, dJ417M14.2, A930010O05Rik, rp62, xmak, MGC82717, MGC146434
Reactivity
Human
(31), (19), (17), (1), (1), (1), (1)
Host
Mouse
(30), (4)
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), Western Blotting (WB)
(32), (22), (5), (4), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393408
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen MAK (AAH39825, 358 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4E9
Isotype IgG Mix kappa
Specificity MAK (AAH39825, 358 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAK
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 70581 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Male Germ Cell-Associated Kinase (MAK) antibody Immunofluorescence of monoclonal antibody to MAK on HeLa cell (antibody concentration 10 µg/mL). Western Blot analysis of MAK expression in transfected 293T cell line by MAK monoclonal antibody (M02), clone 4E9. Lane 1: MAK transfected lysate(52.6 KDa). Lane 2: Non-transfected lysate.
anti-Male Germ Cell-Associated Kinase (MAK) antibody (2) anti-Male Germ Cell-Associated Kinase (MAK) antibody (Image 2)
Background publications Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Mungall, Palmer, Sims et al.: "The DNA sequence and analysis of human chromosome 6...." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Ma, Xia, Desai et al.: "Male germ cell-associated kinase, a male-specific kinase regulated by androgen, is a coactivator of androgen receptor in prostate cancer cells." in: Cancer research, Vol. 66, Issue 17, pp. 8439-47, 2006 (PubMed).

Fu, Larson, Chitta et al.: "Identification of yin-yang regulators and a phosphorylation consensus for male germ cell-associated kinase (MAK)-related kinase." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8639-54, 2006 (PubMed).

Hosts (30), (4)
Reactivities (31), (19), (17), (1), (1), (1), (1)
Applications (32), (22), (5), (4), (2), (1)
Epitopes (6), (5), (5), (2), (2), (1), (1), (1)
back to top