Asparagine-Linked Glycosylation 8, alpha-1,3-Glucosyltransferase Homolog (S. Cerevisiae) (ALG8) antibody

Antigen: Asparagine-Linked Glycosylation 8, alpha-1,3-Glucosyltransferase Homolog (S. Cerevisiae) (ALG8)

Synonyms: MGC2840, MGC142391

Clonality: Monoclonal (2E10)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393411

Additional Information

Alternative name: ALG8

Gene ID: 79053

UniProt: NP_076984

Immunogen: ALG8 (NP_076984, 260 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2E10

Description: Other names: MGC2840 asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ALG8 (NP_076984, 260 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 60088 DA

Comments:

Background: This gene encodes a member of the ALG6/ALG8 glucosyltransferase family. The encoded protein catalyzes the addition of the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation of proteins. Mutations in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ih). Alternatively spliced transcript variants encoding different isoforms have been identified.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged ALG8 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Otsuki, Ota, Nishikawa et al.: "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 12, Issue 2, pp. 117-26, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Stölting, Omran, Erlekotte et al.: "Novel ALG8 mutations expand the clinical spectrum of congenital disorder of glycosylation type Ih." in: Molecular genetics and metabolism, Vol. 98, Issue 3, pp. 305-9, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).