WNK Lysine Deficient Protein Kinase 2 (WNK2) antibody

Antigen: WNK Lysine Deficient Protein Kinase 2 (WNK2)

Synonyms: PRKWNK2, KIAA1760, NY-CO-43, SDCCAG43, P/OKcl.13, ESTM15, X83337, AW122246, mKIAA1760, 1810073P09Rik, RGD1307284, WNK2

Clonality: Monoclonal (2E10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393413

Additional Information

Alternative name: WNK2

Gene ID: 65268

UniProt: NP_006639

Immunogen: WNK2 (NP_006639, 2118 a.a. ~ 2218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2E10

Description: Other names: KIAA1760, NYCO43, P/OKcl.13, PRKWNK2, SDCCAG43WNK lysine deficient protein kinase 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: WNK2 (NP_006639, 2118 a.a. ~ 2218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 242676 DA

Comments:

Background: The protein encoded by this gene is a cytoplasmic serine-threonine kinase that contains cysteine in place of the lysine found at the conserved ATP-binding location in subdomain II of protein kinases. Since this protein does have kinase activity, it is possible that another lysine in the kinase subdomain I can substitute for the missing conserved lysine.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to WNK2 ( Cat # AT4541a ) on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml] Detection limit for recombinant GST tagged WNK2 is approximately 0.1ng/ml as a capture antibody.

anti-WNK Lysine Deficient Protein Kinase 2 (WNK2) antibody (Image 2)

Publications

Product: Jun, Hong, Lal et al.: "Epigenetic silencing of the kinase tumor suppressor WNK2 is tumor-type and tumor-grade specific." in: Neuro-oncology, Vol. 11, Issue 4, pp. 414-22, 2009 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of gene polymorphisms with chronic kidney disease in Japanese individuals." in: International journal of molecular medicine, Vol. 24, Issue 4, pp. 539-47, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).