Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (Met11) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5)

Synonyms: MEK5, MAPKK5, PRKMK5, HsT17454, Mapkk5, Prkmk5, AI324775, AI428457, Mek5, map2k5, fb73f02, MGC172137, wu:fb73f02

Binding Site: Met11

Clonality: Monoclonal (3E10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393416

Additional Information

Alternative name: MAP2K5

Gene ID: 5607

Immunogen: MAP2K5 (AAH08838, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3E10

Description: Other names: HsT17454, MAPKK5, MEK5, PRKMK5 mitogen-activated protein kinase kinase 5

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MAP2K5 (AAH08838, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 50112 DA

Comments:

Background: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to MAP2K5 ( Cat# AT2772a ) on HeLa cell . [antibody concentration 10 ug/ml] MAP2K5 monoclonal antibody (M11), clone 3E10 Western Blot analysis of MAP2K5 expression in Jurkat ( Cat # L017V1 ).

anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (Met11) antibody (Image 2)

Publications

Product: Spiering, Schmolke, Ohnesorge et al.: "MEK5/ERK5 signaling modulates endothelial cell migration and focal contact turnover." in: The Journal of biological chemistry, Vol. 284, Issue 37, pp. 24972-80, 2009 (PubMed).

Rivière, Xiong, Levchenko et al.: "Association of intronic variants of the BTBD9 gene with Tourette syndrome." in: Archives of neurology, Vol. 66, Issue 10, pp. 1267-72, 2009 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Kripke, Shadan, Dawson et al.: "Genotyping sleep disorders patients." in: Psychiatry investigation, Vol. 7, Issue 1, pp. 36-42, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).