|+1 404 474 4654|
|+1 888 205 9894 (TF)|
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||AKT1 (AAH00479, 1 a.a. ~ 481 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: MGC99656, PKB, PRKBA, RAC, RACALPHA v-akt murine thymoma viral oncogene homolog|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||AKT1 (AAH00479, 1 a.a. ~ 481 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||55686 DA|
Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
|Synonyms||AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006|
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure|
|Restrictions||For Research Use only|
Benesch, Schneider, Voelker et al.: "The clinicopathological and prognostic relevance of pyruvate kinase M2 and pAkt expression in breast cancer." in: Anticancer research, Vol. 30, Issue 5, pp. 1689-94, 2010 (PubMed).
Slattery, Herrick, Lundgreen et al.: "Genetic variation in a metabolic signaling pathway and colon and rectal cancer risk: mTOR, PTEN, STK11, RPKAA1, PRKAG2, TSC1, TSC2, PI3K and Akt1." in: Carcinogenesis, Vol. 31, Issue 9, pp. 1604-11, 2010 (PubMed).
Lin, Lin, Yu: "miR-149* induces apoptosis by inhibiting Akt1 and E2F1 in human cancer cells." in: Molecular carcinogenesis, Vol. 49, Issue 8, pp. 719-27, 2010 (PubMed).
Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).
Izquierdo, Truan, Mengual et al.: "HER-2/AKT expression in upper urinary tract urothelial carcinoma: prognostic implications." in: Anticancer research, Vol. 30, Issue 6, pp. 2439-45, 2010 (PubMed).