tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) (AA 1-392) antibody

Details for Product No. ABIN393425
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Antigen
Synonyms MGC89267, DMNT2, DNMT2, MHSAIIP, PUMET, RNMT1, Dnmt2, Rnmt2, dnmt2
Epitope
AA 1-392
(11), (7), (6), (6), (6), (6), (4), (3), (1), (1), (1)
Reactivity
Human
(61), (30), (14), (13), (13), (1), (1), (1)
Host
Mouse
(56), (14)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(53), (35), (18), (11), (10), (7), (6), (3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393425
450.00 $
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Immunogen DNMT2 (AAH47733, 1 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E12
Isotype IgG1 lambda
Specificity DNMT2 (AAH47733, 1 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DNMT2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 44597 DA
Gene ID 12406
Research Area Signaling, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) (AA 1-392) antibody Immunofluorescence of monoclonal antibody to DNMT2 on HeLa cell (antibody concentration 40 µg/mL). Western Blot analysis of DNMT2 expression in transfected 293T cell line by DNMT2 monoclonal antibody (M01), clone 1E12. Lane 1: DNMT2 transfected lysate(45 KDa). Lane 2: Non-transfected lysate.
anti-tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) (AA 1-392) antibody (2) anti-tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) (AA 1-392) antibody (Image 2)
Background publications El-Maarri, Kareta, Mikeska et al.: "A systematic search for DNA methyltransferase polymorphisms reveals a rare DNMT3L variant associated with subtelomeric hypomethylation." in: Human molecular genetics, Vol. 18, Issue 10, pp. 1755-68, 2009 (PubMed).

Halaschek-Wiener, Amirabbasi-Beik, Monfared et al.: "Genetic variation in healthy oldest-old." in: PLoS ONE, Vol. 4, Issue 8, pp. e6641, 2009 (PubMed).

Schaefer, Hagemann, Hanna et al.: "Azacytidine inhibits RNA methylation at DNMT2 target sites in human cancer cell lines." in: Cancer research, Vol. 69, Issue 20, pp. 8127-32, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Vivekanandan, Daniel, Kannangai et al.: "Hepatitis B virus replication induces methylation of both host and viral DNA." in: Journal of virology, Vol. 84, Issue 9, pp. 4321-9, 2010 (PubMed).

Validation Images
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