tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) antibody

Antigen: tRNA Aspartic Acid Methyltransferase 1 (TRDMT1)

Synonyms: ecat7, D6Ertd14e, MGC102308, DNMT3L, MGC53207, MGC89267

Clonality: Monoclonal (1E12)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393425

Additional Information

Alternative name: DNMT2

Gene ID: 1787

Immunogen: DNMT2 (AAH47733, 1 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 lambda

Clone: 1E12

Description: Other names: M.HsaIIP, PuMet DNA (cytosine-5-)-methyltransferase 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DNMT2 (AAH47733, 1 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 44597 DA

Comments:

Background: This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to DNMT2 (AT1806a) on HeLa cell . [antibody concentration 40 ug/ml] Western Blot analysis of DNMT2 expression in transfected 293T cell line by DNMT2 monoclonal antibody (M01), clone 1E12. Lane 1: DNMT2 transfected lysate(45 KDa). Lane 2: Non-transfected lysate.

anti-tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) antibody (Image 2)

Publications

Product: El-Maarri, Kareta, Mikeska et al.: "A systematic search for DNA methyltransferase polymorphisms reveals a rare DNMT3L variant associated with subtelomeric hypomethylation." in: Human molecular genetics, Vol. 18, Issue 10, pp. 1755-68, 2009 (PubMed).

Halaschek-Wiener, Amirabbasi-Beik, Monfared et al.: "Genetic variation in healthy oldest-old." in: PLoS ONE, Vol. 4, Issue 8, pp. e6641, 2009 (PubMed).

Schaefer, Hagemann, Hanna et al.: "Azacytidine inhibits RNA methylation at DNMT2 target sites in human cancer cell lines." in: Cancer research, Vol. 69, Issue 20, pp. 8127-32, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Vivekanandan, Daniel, Kannangai et al.: "Hepatitis B virus replication induces methylation of both host and viral DNA." in: Journal of virology, Vol. 84, Issue 9, pp. 4321-9, 2010 (PubMed).