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Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1654-1742) antibody

Details for Product No. ABIN393429, Supplier: Log in to see
Antigen
  • CG9594
  • CHD-3
  • CHD3
  • DmCHD3
  • Dmel\\CG9594
  • chd3
  • dCHD3
  • LOC568230
  • zfh
  • mi-2a
  • mi2-alpha
  • Mi-2a
  • Mi2-ALPHA
  • ZFH
  • 2600010P09Rik
  • AF020312
  • Chd7
  • Prp7
  • Prp9-1
Epitope
AA 1654-1742
6
5
5
3
2
2
2
2
1
1
1
1
Reactivity
Human
57
30
17
2
1
1
Host
Mouse
32
31
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
41
23
16
16
13
11
11
11
6
3
2
1
Supplier
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Immunogen CHD3 (NP_001005273, 1654 a.a. ~ 1742 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5E12
Isotype IgG2a kappa
Specificity CHD3 (NP_001005273, 1654 a.a. ~ 1742 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CHD3 (CHD3 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 226592 DA
Gene ID 12406
NCBI Accession NP_001005273
Research Area Signaling, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) (AA 1654-1742) antibody (ABIN393429) Immunoperoxidase of monoclonal antibody to CHD3 on formalin-fixed paraffin-embedded h...
Background publications Wang, Zhang, Chen et al.: "LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer." in: Cell, Vol. 138, Issue 4, pp. 660-72, 2009 (PubMed).

Burd, Kinyamu, Miller et al.: "UV radiation regulates Mi-2 through protein translation and stability." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34976-82, 2008 (PubMed).

Sun, Chen, Davie: "Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin." in: The Journal of biological chemistry, Vol. 282, Issue 45, pp. 33227-36, 2007 (PubMed).

Sillibourne, Delaval, Redick et al.: "Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity." in: Molecular biology of the cell, Vol. 18, Issue 9, pp. 3667-80, 2007 (PubMed).

Pastorcic, Das: "The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene." in: The FEBS journal, Vol. 274, Issue 6, pp. 1434-48, 2007 (PubMed).