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Details for Product No. ABIN393429

Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody

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Antigen
Synonyms ZFH, Mi-2a, Mi2-ALPHA, 2600010P09Rik, AF020312, Chd7, Prp7, Prp9-1, CG9594, CHD-3, CHD3, DmCHD3, Dmel\\CG9594, chd3, dCHD3, LOC568230, zfh, mi-2a, mi2-alpha
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393429
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen CHD3 (NP_001005273, 1654 a.a. ~ 1742 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5E12
Isotype IgG2a kappa
Specificity CHD3 (NP_001005273, 1654 a.a. ~ 1742 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CHD3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 226592 DA
Gene ID 12406
UniProt NP_001005273
Research Area Signaling, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Pastorcic, Das: "The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene." in: The FEBS journal, Vol. 274, Issue 6, pp. 1434-48, 2007 (PubMed).

Sillibourne, Delaval, Redick et al.: "Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity." in: Molecular biology of the cell, Vol. 18, Issue 9, pp. 3667-80, 2007 (PubMed).

Sun, Chen, Davie: "Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin." in: The Journal of biological chemistry, Vol. 282, Issue 45, pp. 33227-36, 2007 (PubMed).

Burd, Kinyamu, Miller et al.: "UV radiation regulates Mi-2 through protein translation and stability." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34976-82, 2008 (PubMed).

Wang, Zhang, Chen et al.: "LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer." in: Cell, Vol. 138, Issue 4, pp. 660-72, 2009 (PubMed).

Alternatives for antigen "Chromodomain Helicase DNA Binding Protein 3 (CHD3)", type "Antibodies"
Hosts (20), (16)
Reactivities (34), (20), (12), (12), (12), (1)
Applications (20), (18), (9), (8), (8), (7), (6), (6), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (1), (1), (1), (1)
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