Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody

Antigen: Chromodomain Helicase DNA Binding Protein 3 (CHD3)

Synonyms: ZFH, Mi-2a, Mi2-ALPHA, Chd7, Prp7, Prp9-1, AF020312, MGC40857, 2600010P09Rik, CHD3, LOC568230, zfh, mi-2a, mi2-alpha

Clonality: Monoclonal (5E12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393429

Additional Information

Alternative name: CHD3

Gene ID: 1107

UniProt: NP_001005273

Immunogen: CHD3 (NP_001005273, 1654 a.a. ~ 1742 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 5E12

Description: Other names: Mi2a, Mi2ALPHA, ZFH chromodomain helicase DNA binding protein 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CHD3 (NP_001005273, 1654 a.a. ~ 1742 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 226592 DA

Comments:

Background: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to CHD3 ( Cat # AT1513a ) on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Publications

Product: Pastorcic, Das: "The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene." in: The FEBS journal, Vol. 274, Issue 6, pp. 1434-48, 2007 (PubMed).

Sillibourne, Delaval, Redick et al.: "Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity." in: Molecular biology of the cell, Vol. 18, Issue 9, pp. 3667-80, 2007 (PubMed).

Sun, Chen, Davie: "Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin." in: The Journal of biological chemistry, Vol. 282, Issue 45, pp. 33227-36, 2007 (PubMed).

Burd, Kinyamu, Miller et al.: "UV radiation regulates Mi-2 through protein translation and stability." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34976-82, 2008 (PubMed).

Wang, Zhang, Chen et al.: "LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer." in: Cell, Vol. 138, Issue 4, pp. 660-72, 2009 (PubMed).