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NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody

Details for Product No. ABIN393431
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Antigen
Synonyms NCK1, NCK, NCKalpha, nck-1, 6330586M15Rik, D230010O13Rik, Nck, Nck-1
Epitope
AA 185-294
(17), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(53), (10), (9), (4)
Host
Mouse
(38), (15), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(53), (28), (21), (8), (8), (5), (4), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393431
450.00 $
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Immunogen NCK1 (AAH06403, 185 a.a. ~ 294 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1A1
Isotype IgG Mix kappa
Specificity NCK1 (AAH06403, 185 a.a. ~ 294 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NCK1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 42864 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody NCK1 monoclonal antibody (M01), clone 1A1 Western Blot analysis of NCK1 expression in HeLa NE. Detection limit for recombinant GST tagged NCK1 is approximately 0.1 ng/mL as a capture antibody.
anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody (2) anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody (Image 2)
Background publications Voss, Lettau, Janssen: "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." in: BMC immunology, Vol. 10, pp. 53, 2009 (PubMed).

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010 (PubMed).

Gehmlich, Hayess, Legler et al.: "Ponsin interacts with Nck adapter proteins: implications for a role in cytoskeletal remodelling during differentiation of skeletal muscle cells." in: European journal of cell biology, Vol. 89, Issue 5, pp. 351-64, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Barda-Saad, Shirasu, Pauker et al.: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010 (PubMed).

Validation Images
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