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RNF125 antibody (Ring Finger Protein 125) (AA 143-232)

Details for Product anti-RNF125 Antibody No. ABIN393443, Supplier: Log in to see
Antigen
  • 4930553F04Rik
  • TRAC-1
  • TRAC1
Epitope
AA 143-232
4
3
3
3
2
2
2
2
1
Reactivity
Human
48
28
26
4
4
4
2
2
1
1
1
1
Host
Mouse
39
6
5
Clonality (Clone)
Monoclonal ()
Conjugate
This RNF125 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
30
19
11
8
4
3
1
1
Supplier
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Immunogen RNF125 (NP_060301, 143 a.a. ~ 232 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1A5
Isotype IgG1 kappa
Specificity RNF125 (NP_060301, 143 a.a. ~ 232 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF125 (RNF125 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 26454 DA
Gene ID 12406
NCBI Accession NP_060301
Research Area Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes a novel E3 ubiquitin ligase that contains an N-terminal RING finger domain. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-RNF125 antibody (Ring Finger Protein 125) (AA 143-232) (ABIN393443) Detection limit for recombinant GST tagged RNF125 is approximately 0.03 ng/mL as a ca...
Background publications Giannini, Gao, Bijlmakers: "T-cell regulator RNF125/TRAC-1 belongs to a novel family of ubiquitin ligases with zinc fingers and a ubiquitin-binding domain." in: The Biochemical journal, Vol. 410, Issue 1, pp. 101-11, 2008

Arimoto, Konishi, Shimotohno: "UbcH8 regulates ubiquitin and ISG15 conjugation to RIG-I." in: Molecular immunology, Vol. 45, Issue 4, pp. 1078-84, 2007

Shoji-Kawata, Zhong, Kameoka, Iwabu, Sapsutthipas, Luftig, Ikuta: "The RING finger ubiquitin ligase RNF125/TRAC-1 down-modulates HIV-1 replication in primary human peripheral blood mononuclear cells." in: Virology, Vol. 368, Issue 1, pp. 191-204, 2007

Zhao, Li, Pardo, Chu, Liao, Huang, Dong, Zhou, Huang, Huang, Bennett, Molineaux, Lu, Daniel-Issakani, Payan, Masuda: "A novel E3 ubiquitin ligase TRAC-1 positively regulates T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 9, pp. 5288-97, 2005

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004