Ring Finger Protein 125 (RNF125) antibody

Antigen: Ring Finger Protein 125 (RNF125)

Synonyms: TRAC1, FLJ20456, MGC21737

Clonality: Monoclonal (1A5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393443

Additional Information

Alternative name: RNF125

Gene ID: 54941

UniProt: NP_060301

Immunogen: RNF125 (NP_060301, 143 a.a. ~ 232 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1A5

Description: Other names: FLJ20456, MGC21737 ring finger protein 125

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RNF125 (NP_060301, 143 a.a. ~ 232 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 26454 DA

Comments:

Background: This gene encodes a novel E3 ubiquitin ligase that contains an N-terminal RING finger domain. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged RNF125 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Zhao, Li, Pardo et al.: "A novel E3 ubiquitin ligase TRAC-1 positively regulates T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 9, pp. 5288-97, 2005 (PubMed).

Shoji-Kawata, Zhong, Kameoka et al.: "The RING finger ubiquitin ligase RNF125/TRAC-1 down-modulates HIV-1 replication in primary human peripheral blood mononuclear cells." in: Virology, Vol. 368, Issue 1, pp. 191-204, 2007 (PubMed).

Arimoto, Konishi, Shimotohno: "UbcH8 regulates ubiquitin and ISG15 conjugation to RIG-I." in: Molecular immunology, Vol. 45, Issue 4, pp. 1078-84, 2007 (PubMed).

Giannini, Gao, Bijlmakers: "T-cell regulator RNF125/TRAC-1 belongs to a novel family of ubiquitin ligases with zinc fingers and a ubiquitin-binding domain." in: The Biochemical journal, Vol. 410, Issue 1, pp. 101-11, 2008 (PubMed).