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WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) (AA 152-261) antibody

Details for Product No. ABIN393446, Supplier: Log in to see
Antigen
  • wu:fb30b12
  • wwp1
  • zgc:66401
  • WWP1
  • aip5
  • tiul1
  • hsdrp1
  • AIP5
  • Tiul1
  • hSDRP1
  • 8030445B08Rik
  • SDRP1
Epitope
AA 152-261
4
4
3
2
1
1
1
1
Reactivity
Human
26
8
8
1
1
1
Host
Mouse
19
7
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
Application
Immunofluorescence (IF), Western Blotting (WB)
22
11
4
3
2
2
1
Supplier
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Immunogen WWP1 (NP_008944, 152 a.a. ~ 261 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1A7
Isotype IgG2a kappa
Specificity WWP1 (NP_008944, 152 a.a. ~ 261 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name WWP1 (WWP1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 105202 DA
Gene ID 12406
NCBI Accession NP_008944
Research Area Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants, however, the full length nature of these transcripts has not been defined.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) (AA 152-261) antibody (ABIN393446) Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) (AA 152-261) antibody (ABIN393446) anti-WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) (AA 152-261) antibody (Image 2)
Background publications Edwards, Clowes, Tsang et al.: "Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5." in: The Biochemical journal, Vol. 423, Issue 1, pp. 31-9, 2009 (PubMed).

Li, Zhou, Alimandi et al.: "WW domain containing E3 ubiquitin protein ligase 1 targets the full-length ErbB4 for ubiquitin-mediated degradation in breast cancer." in: Oncogene, Vol. 28, Issue 33, pp. 2948-58, 2009 (PubMed).

Xin, Rual, Hirozane-Kishikawa et al.: "Shifted Transversal Design smart-pooling for high coverage interactome mapping." in: Genome research, Vol. 19, Issue 7, pp. 1262-9, 2009 (PubMed).

Eastman, Yassaee, Bieniasz: "A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover." in: The Journal of cell biology, Vol. 184, Issue 6, pp. 881-94, 2009 (PubMed).

Chen, Zhou, Sheehan et al.: "Overexpression of WWP1 is associated with the estrogen receptor and insulin-like growth factor receptor 1 in breast carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 124, Issue 12, pp. 2829-36, 2009 (PubMed).