WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) (AA 152-261) antibody

Details for Product No. ABIN393446
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Antigen
Synonyms wu:fb30b12, wwp1, zgc:66401, WWP1, aip5, tiul1, hsdrp1, AIP5, Tiul1, hSDRP1, 8030445B08Rik, SDRP1
Epitope
AA 152-261
(4), (3), (3), (1), (1), (1)
Reactivity
Human
(31), (16), (16), (13), (13), (1)
Host
Mouse
(26), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(21), (11), (10), (4), (4), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393446
450.00 $
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Immunogen WWP1 (NP_008944, 152 a.a. ~ 261 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1A7
Isotype IgG2a kappa
Specificity WWP1 (NP_008944, 152 a.a. ~ 261 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name WWP1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 105202 DA
Gene ID 12406
NCBI Accession NP_008944
Research Area Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants, however, the full length nature of these transcripts has not been defined.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) (AA 152-261) antibody Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell (antibody concentration 10 µg/mL). WWP1 monoclonal antibody (M01), clone 1A7 Western Blot analysis of WWP1 expression in A-431.
anti-WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) (AA 152-261) antibody (2) anti-WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) (AA 152-261) antibody (Image 2)
Background publications Chen, Zhou, Sheehan et al.: "Overexpression of WWP1 is associated with the estrogen receptor and insulin-like growth factor receptor 1 in breast carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 124, Issue 12, pp. 2829-36, 2009 (PubMed).

Eastman, Yassaee, Bieniasz: "A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover." in: The Journal of cell biology, Vol. 184, Issue 6, pp. 881-94, 2009 (PubMed).

Xin, Rual, Hirozane-Kishikawa et al.: "Shifted Transversal Design smart-pooling for high coverage interactome mapping." in: Genome research, Vol. 19, Issue 7, pp. 1262-9, 2009 (PubMed).

Li, Zhou, Alimandi et al.: "WW domain containing E3 ubiquitin protein ligase 1 targets the full-length ErbB4 for ubiquitin-mediated degradation in breast cancer." in: Oncogene, Vol. 28, Issue 33, pp. 2948-58, 2009 (PubMed).

Edwards, Clowes, Tsang et al.: "Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5." in: The Biochemical journal, Vol. 423, Issue 1, pp. 31-9, 2009 (PubMed).

Validation Images
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