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Details for Product No. ABIN393446

WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) antibody

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Antigen
Synonyms AIP5, Tiul1, hSDRP1, SDRP1, 8030445B08Rik, wu:fb30b12, wwp1, zgc:66401, WWP1, aip5, tiul1, hsdrp1
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393446
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen WWP1 (NP_008944, 152 a.a. ~ 261 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1A7
Isotype IgG2a kappa
Specificity WWP1 (NP_008944, 152 a.a. ~ 261 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name WWP1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 105202 DA
Gene ID 12406
UniProt NP_008944
Research Area Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence
Comment

Background: WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants, however, the full length nature of these transcripts has not been defined.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Chen, Zhou, Sheehan et al.: "Overexpression of WWP1 is associated with the estrogen receptor and insulin-like growth factor receptor 1 in breast carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 124, Issue 12, pp. 2829-36, 2009 (PubMed).

Eastman, Yassaee, Bieniasz: "A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover." in: The Journal of cell biology, Vol. 184, Issue 6, pp. 881-94, 2009 (PubMed).

Xin, Rual, Hirozane-Kishikawa et al.: "Shifted Transversal Design smart-pooling for high coverage interactome mapping." in: Genome research, Vol. 19, Issue 7, pp. 1262-9, 2009 (PubMed).

Li, Zhou, Alimandi et al.: "WW domain containing E3 ubiquitin protein ligase 1 targets the full-length ErbB4 for ubiquitin-mediated degradation in breast cancer." in: Oncogene, Vol. 28, Issue 33, pp. 2948-58, 2009 (PubMed).

Edwards, Clowes, Tsang et al.: "Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5." in: The Biochemical journal, Vol. 423, Issue 1, pp. 31-9, 2009 (PubMed).

Alternatives for antigen "WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1)", type "Antibodies"
Hosts (22), (5)
Reactivities (26), (15), (15), (13), (13), (1)
Applications (18), (14), (10), (4), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (4), (2), (1)
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