F-Box Protein 8 (FBXO8) antibody

Antigen: F-Box Protein 8 (FBXO8)

Synonyms: FBS, DC10, FBX8, Fbx8, MGC84680, FBXO8, MGC136842, zgc:136842

Clonality: Monoclonal (1C11)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393472

Additional Information

Alternative name: FBXO8

Gene ID: 26269

UniProt: NP_036312

Immunogen: FBXO8 (NP_036312, 1 a.a. ~ 78 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1C11

Description: Other names: DC10, FBS, FBX8 F-box protein 8

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: FBXO8 (NP_036312, 1 a.a. ~ 78 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 37068 DA

Comments:

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It contains a C-terminal amino acid sequence that bears a significant similarity with a portion of yeast Sec7p, a critical regulator of vesicular protein transport. This human protein may interact with ADP-ribosylation factor(s)(ARFs) and exhibit ARF-GEF (guanine nucleotide exchange factor) activity.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged FBXO8 is approximately 0.1ng/ml as a capture antibody.

Publications

Product: Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Cronin, Tomik, Bradley et al.: "Screening for replication of genome-wide SNP associations in sporadic ALS." in: European journal of human genetics : EJHG, Vol. 17, Issue 2, pp. 213-8, 2009 (PubMed).