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Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1) (AA 1-171) antibody

Details for Product No. ABIN393474
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Antigen
Synonyms e217k, ubc7, ube2g, UB2G1, E217K, UBC7, UBE2G, 2700059C12Rik, AI256795, AU014992, AW552068, D130023C12Rik, Ubc7
Epitope
AA 1-171
(12), (8), (7), (3), (2), (2), (1), (1)
Reactivity
Human
(31), (20), (15), (12), (12)
Host
Mouse
(40), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(34), (22), (10), (9), (7), (3), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393474
450.00 $
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Immunogen UBE2G1 (AAH02775, 1 a.a. ~ 171 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1C12-1B2
Isotype IgG1 kappa
Specificity UBE2G1 (AAH02775, 1 a.a. ~ 171 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2G1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 19509 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1) (AA 1-171) antibody Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell (antibody concentration 20 µg/mL). UBE2G1 monoclonal antibody (M01), clone 1C12-1B2 Western Blot analysis of UBE2G1 expression in Hela.
anti-Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1) (AA 1-171) antibody (2) anti-Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1) (AA 1-171) antibody (Image 2)
Background publications Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Kim, Zavacki, Curcio-Morelli et al.: "Endoplasmic reticulum-associated degradation of the human type 2 iodothyronine deiodinase (D2) is mediated via an association between mammalian UBC7 and the carboxyl region of D2." in: Molecular endocrinology (Baltimore, Md.), Vol. 17, Issue 12, pp. 2603-12, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Sagar, Gereben, Callebaut et al.: "Ubiquitination-induced conformational change within the deiodinase dimer is a switch regulating enzyme activity." in: Molecular and cellular biology, Vol. 27, Issue 13, pp. 4774-83, 2007 (PubMed).

Validation Images
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