Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1)

Synonyms: UBC7, E217K, UBE2G, ubc7, e217k, ube2g, MGC75869, UB2G1

Clonality: Monoclonal (1C12-1B2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393474

Additional Information

Alternative name: UBE2G1

Gene ID: 7326

Immunogen: UBE2G1 (AAH02775, 1 a.a. ~ 171 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1C12-1B2

Description: Other names: E217K, UBC7, UBE2Gubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: UBE2G1 (AAH02775, 1 a.a. ~ 171 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 19509 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to UBE2G1 (AT4435a) on HeLa cell. [antibody concentration 20ug/ml] UBE2G1 monoclonal antibody (M01), clone 1C12-1B2 Western Blot analysis of UBE2G1 expression in Hela ( Cat # L013V1 ).

anti-Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1) antibody (Image 2)

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Kim, Zavacki, Curcio-Morelli et al.: "Endoplasmic reticulum-associated degradation of the human type 2 iodothyronine deiodinase (D2) is mediated via an association between mammalian UBC7 and the carboxyl region of D2." in: Molecular endocrinology (Baltimore, Md.), Vol. 17, Issue 12, pp. 2603-12, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Sagar, Gereben, Callebaut et al.: "Ubiquitination-induced conformational change within the deiodinase dimer is a switch regulating enzyme activity." in: Molecular and cellular biology, Vol. 27, Issue 13, pp. 4774-83, 2007 (PubMed).