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A Kinase (PRKA) Anchor Protein 12 (AKAP12) (AA 1675-1783) antibody

Details for Product No. ABIN393479
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Antigen
Synonyms akap12, cb27, esr1, fb72g12, id:ibd1202, sb:cb27, si:ch73-192g21.1, wu:fb72g12, wu:fc21c03, AKAP12, AI317366, SSeCKS, Srcs5, Tsga12, AKAP12A, AKAP12B, AKAP12G, AKAP250
Epitope
AA 1675-1783
(3), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(28), (17), (14), (12), (2)
Host
Mouse
(22), (5), (4), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(18), (12), (10), (7), (6), (5), (5), (3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393479
450.00 $
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Immunogen AKAP12 (NP_005091, 1675 a.a. ~ 1783 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1C5
Isotype IgG2b kappa
Specificity AKAP12 (NP_005091, 1675 a.a. ~ 1783 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKAP12
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 191483 DA
Gene ID 12406
UniProt NP_005091
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-A Kinase (PRKA) Anchor Protein 12 (AKAP12) (AA 1675-1783) antibody Detection limit for recombinant GST tagged AKAP12 is approximately 0.03 ng/mL as a capture antibody.
Background publications Yan, Walkiewicz, Carlson et al.: "Gravin dynamics regulates the subcellular distribution of PKA." in: Experimental cell research, Vol. 315, Issue 7, pp. 1247-59, 2009 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of gene polymorphisms with chronic kidney disease in Japanese individuals." in: International journal of molecular medicine, Vol. 24, Issue 4, pp. 539-47, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Su, Bu, Engelberg et al.: "SSeCKS/Gravin/AKAP12 inhibits cancer cell invasiveness and chemotaxis by suppressing a protein kinase C- Raf/MEK/ERK pathway." in: The Journal of biological chemistry, Vol. 285, Issue 7, pp. 4578-86, 2010 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).

Validation Images
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