A Kinase (PRKA) Anchor Protein 12 (AKAP12) antibody

Antigen: A Kinase (PRKA) Anchor Protein 12 (AKAP12)

Synonyms: AKAP250, FLJ20945, FLJ97621, DKFZp686M0430, DKFZp686O0331, Srcs5, SSeCKS, Tsga12, AI317366, AKAP12A, AKAP12B, AKAP12G, AKAP12, esr1, sb:cb27, wu:fb72g12, wu:fc21c03, si:ch73-192g21.1

Clonality: Monoclonal (1C5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393479

Additional Information

Alternative name: AKAP12

Gene ID: 9590

UniProt: NP_005091

Immunogen: AKAP12 (NP_005091, 1675 a.a. ~ 1783 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 1C5

Description: Other names: AKAP250, DKFZp686M0430, DKFZp686O0331 A kinase (PRKA) anchor protein (gravin) 12

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AKAP12 (NP_005091, 1675 a.a. ~ 1783 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 191483 DA

Comments:

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged AKAP12 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Yan, Walkiewicz, Carlson et al.: "Gravin dynamics regulates the subcellular distribution of PKA." in: Experimental cell research, Vol. 315, Issue 7, pp. 1247-59, 2009 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of gene polymorphisms with chronic kidney disease in Japanese individuals." in: International journal of molecular medicine, Vol. 24, Issue 4, pp. 539-47, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Su, Bu, Engelberg et al.: "SSeCKS/Gravin/AKAP12 inhibits cancer cell invasiveness and chemotaxis by suppressing a protein kinase C- Raf/MEK/ERK pathway." in: The Journal of biological chemistry, Vol. 285, Issue 7, pp. 4578-86, 2010 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).