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RNF20 antibody (Ring Finger Protein 20) (AA 177-277)

Details for Product anti-RNF20 Antibody No. ABIN393482, Supplier: Log in to see
Antigen
  • 4833430L21Rik
  • AW540162
  • BRE1
  • BRE1A
  • C79397
  • hBRE1
  • mKIAA4116
Epitope
AA 177-277
23
15
5
3
3
3
3
2
2
2
1
1
Reactivity
Human
69
26
24
2
2
2
Host
Mouse
62
7
Clonality (Clone)
Monoclonal ()
Conjugate
This RNF20 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
53
26
12
11
8
7
2
1
Supplier
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Immunogen RNF20 (NP_062538, 177 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1C6
Isotype IgG2a kappa
Specificity RNF20 (NP_062538, 177 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF20 (RNF20 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 113662 DA
Gene ID 12406
NCBI Accession NP_062538
Research Area Ubiquitin-related antibodies, Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3.

Restrictions For Research Use only
Concentration 1mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-RNF20 antibody (Ring Finger Protein 20) (AA 177-277) (ABIN393482) Detection limit for recombinant GST tagged RNF20 is approximately 0.03 ng/mL as a cap...
Background publications Svendsen, Smogorzewska, Sowa, OConnell, Gygi, Elledge, Harper: "Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair." in: Cell, Vol. 138, Issue 1, pp. 63-77, 2009

Kim, Guermah, McGinty, Lee, Tang, Milne, Shilatifard, Muir, Roeder: "RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells." in: Cell, Vol. 137, Issue 3, pp. 459-71, 2009

Liu, Oh, Okada, Liu, Cheng, Peng, Brat, Sun, Zhou, Gu, Ye: "Human BRE1 is an E3 ubiquitin ligase for Ebp1 tumor suppressor." in: Molecular biology of the cell, Vol. 20, Issue 3, pp. 757-68, 2009

Shema, Tirosh, Aylon, Huang, Ye, Moskovits, Raver-Shapira, Minsky, Pirngruber, Tarcic, Hublarova, Moyal, Gana-Weisz, Shiloh, Yarden, Johnsen, Vojtesek, Berger, Oren: "The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression." in: Genes & development, Vol. 22, Issue 19, pp. 2664-76, 2008

Barber, McManus, Yuen, Reis, Parmigiani, Shen, Barrett, Nouhi, Spencer, Markowitz, Velculescu, Kinzler, Vogelstein, Lengauer, Hieter: "Chromatid cohesion defects may underlie chromosome instability in human colorectal cancers." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3443-8, 2008