Ring Finger Protein 20 (RNF20) (AA 177-277) antibody

Details for Product No. ABIN393482
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Antigen
Synonyms 4833430L21Rik, AW540162, C79397, mKIAA4116, BRE1, BRE1A, hBRE1
Epitope
AA 177-277
(10), (7), (3), (3), (2), (2), (2), (1), (1)
Reactivity
Human
(44), (19), (18), (14), (14), (14), (12)
Host
Mouse
(39), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(33), (13), (10), (8), (6), (6), (3)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393482
450.00 $
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Immunogen RNF20 (NP_062538, 177 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1C6
Isotype IgG2a kappa
Specificity RNF20 (NP_062538, 177 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF20
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 113662 DA
Gene ID 12406
NCBI Accession NP_062538
Research Area Ubiquitin-related antibodies, Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3.

Restrictions For Research Use only
Concentration 1mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ring Finger Protein 20 (RNF20) (AA 177-277) antibody Detection limit for recombinant GST tagged RNF20 is approximately 0.03 ng/mL as a capture antibody.
Background publications Barber, McManus, Yuen et al.: "Chromatid cohesion defects may underlie chromosome instability in human colorectal cancers." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3443-8, 2008 (PubMed).

Shema, Tirosh, Aylon et al.: "The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression." in: Genes & development, Vol. 22, Issue 19, pp. 2664-76, 2008 (PubMed).

Liu, Oh, Okada et al.: "Human BRE1 is an E3 ubiquitin ligase for Ebp1 tumor suppressor." in: Molecular biology of the cell, Vol. 20, Issue 3, pp. 757-68, 2009 (PubMed).

Kim, Guermah, McGinty et al.: "RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells." in: Cell, Vol. 137, Issue 3, pp. 459-71, 2009 (PubMed).

Svendsen, Smogorzewska, Sowa et al.: "Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair." in: Cell, Vol. 138, Issue 1, pp. 63-77, 2009 (PubMed).

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