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Ring Finger Protein 20 (RNF20) (AA 177-277) antibody

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Synonyms 4833430L21Rik, AW540162, C79397, mKIAA4116, BRE1, BRE1A, hBRE1
AA 177-277
(21), (15), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1)
(66), (26), (24), (2), (2), (2)
(59), (7)
Clonality (Clone)
Monoclonal ()
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
ELISA, Western Blotting (WB)
(50), (26), (12), (11), (8), (7), (2), (1)
Pubmed 5 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 8 to 11 Business Days
Immunogen RNF20 (NP_062538, 177 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1C6
Isotype IgG2a kappa
Specificity RNF20 (NP_062538, 177 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF20 (RNF20 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 113662 DA
Gene ID 12406
NCBI Accession NP_062538
Research Area Ubiquitin-related antibodies, Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Background: The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3.

Restrictions For Research Use only
Concentration 1mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-Ring Finger Protein 20 (RNF20) (AA 177-277) antibody (ABIN393482) Detection limit for recombinant GST tagged RNF20 is approximately 0.03 ng/mL as a cap...
Background publications Svendsen, Smogorzewska, Sowa et al.: "Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair." in: Cell, Vol. 138, Issue 1, pp. 63-77, 2009 (PubMed).

Kim, Guermah, McGinty et al.: "RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells." in: Cell, Vol. 137, Issue 3, pp. 459-71, 2009 (PubMed).

Liu, Oh, Okada et al.: "Human BRE1 is an E3 ubiquitin ligase for Ebp1 tumor suppressor." in: Molecular biology of the cell, Vol. 20, Issue 3, pp. 757-68, 2009 (PubMed).

Shema, Tirosh, Aylon et al.: "The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression." in: Genes & development, Vol. 22, Issue 19, pp. 2664-76, 2008 (PubMed).

Barber, McManus, Yuen et al.: "Chromatid cohesion defects may underlie chromosome instability in human colorectal cancers." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3443-8, 2008 (PubMed).

Catalog No. ABIN393482
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