Ring Finger Protein 20 (RNF20) antibody

Antigen: Ring Finger Protein 20 (RNF20)

Synonyms: BRE1, BRE1A, hBRE1, FLJ11189, FLJ20382, KIAA2779, MGC129667, MGC129668

Clonality: Monoclonal (1C6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393482

Additional Information

Alternative name: RNF20

Gene ID: 56254

UniProt: NP_062538

Immunogen: RNF20 (NP_062538, 177 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1C6

Description: Other names: BRE1A, FLJ11189, FLJ20382, KIAA2779ring finger protein 20

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RNF20 (NP_062538, 177 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 113662 DA

Comments:

Background: The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Concentration: 1mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged RNF20 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Barber, McManus, Yuen et al.: "Chromatid cohesion defects may underlie chromosome instability in human colorectal cancers." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3443-8, 2008 (PubMed).

Shema, Tirosh, Aylon et al.: "The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression." in: Genes & development, Vol. 22, Issue 19, pp. 2664-76, 2008 (PubMed).

Liu, Oh, Okada et al.: "Human BRE1 is an E3 ubiquitin ligase for Ebp1 tumor suppressor." in: Molecular biology of the cell, Vol. 20, Issue 3, pp. 757-68, 2009 (PubMed).

Kim, Guermah, McGinty et al.: "RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells." in: Cell, Vol. 137, Issue 3, pp. 459-71, 2009 (PubMed).

Svendsen, Smogorzewska, Sowa et al.: "Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair." in: Cell, Vol. 138, Issue 1, pp. 63-77, 2009 (PubMed).