anti-GADD45G antibody (Growth Arrest and DNA-Damage-Inducible, gamma) (AA 1-160)

Details for Product anti-GADD45G Antibody No. ABIN393489
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AA 1-160
(17), (16), (16), (12), (9), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1)
(165), (25), (22), (2), (2), (2), (2), (1), (1)
(87), (47), (30), (3)
Clonality (Clone)
Monoclonal ()
This GADD45G antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
ELISA, Western Blotting (WB)
(115), (55), (28), (17), (11), (10), (8), (6), (3), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Immunogen GADD45G (AAH19325, 1 a.a. ~ 160 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D3
Isotype IgG2a kappa
Specificity GADD45G (AAH19325, 1 a.a. ~ 160 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name GADD45G (GADD45G Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17121 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways Cell Division Cycle
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-GADD45G antibody (Growth Arrest and DNA-Damage-Inducible, gamma) (AA 1-160) (ABIN393489) Detection limit for recombinant GST tagged GADD45G is approximately 0.03 ng/mL as a c...
Background publications Zhang, Li, Shao et al.: "Semi-quantitative detection of GADD45-gamma methylation levels in gastric, colorectal and pancreatic cancers using methylation-sensitive high-resolution melting analysis." in: Journal of cancer research and clinical oncology, Vol. 136, Issue 8, pp. 1267-73, 2010 (PubMed).

Yu, Cho, Park et al.: "Urinary GADD45gamma expression is associated with progression of lgA nephropathy." in: American journal of nephrology, Vol. 30, Issue 2, pp. 135-9, 2009 (PubMed).

Palmieri, Wilson, Iversen et al.: "Polymorphism in the IL18 gene and epithelial ovarian cancer in non-Hispanic white women. ..." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 17, Issue 12, pp. 3567-72, 2008 (PubMed).

Zhu, Shao, Xu et al.: "Gadd45-alpha and Gadd45-gamma utilize p38 and JNK signaling pathways to induce cell cycle G2/M arrest in Hep-G2 hepatoma cells." in: Molecular biology reports, Vol. 36, Issue 8, pp. 2075-85, 2009 (PubMed).

Zhang, Zhao, Li et al.: "Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma." in: Acta crystallographica. Section F, Structural biology and crystallization communications, Vol. 64, Issue Pt 11, pp. 1070-3, 2008 (PubMed).

Catalog No. ABIN393489
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