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MED8 antibody (Mediator Complex Subunit 8) (AA 1-60)

Details for Product anti-MED8 Antibody No. ABIN393490, Supplier: Log in to see
Antigen
  • 2210021A15Rik
  • AB041805
  • ARC32
  • med8-a
  • MNCb-2386
  • zgc:76877
Epitope
AA 1-60
16
16
10
3
3
3
2
1
1
1
1
Reactivity
Human
34
8
5
1
1
Host
Mouse
28
6
Clonality (Clone)
Monoclonal ()
Conjugate
This MED8 antibody is un-conjugated
2
2
2
2
2
2
Application
ELISA, Western Blotting (WB)
32
24
2
1
Supplier
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Immunogen MED8 (NP_443109, 1 a.a. ~ 60 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D3
Isotype IgG2a kappa
Specificity MED8 (NP_443109, 1 a.a. ~ 60 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MED8 (MED8 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 29080 DA
Gene ID 12406
NCBI Accession NP_443109
Research Area Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-MED8 antibody (Mediator Complex Subunit 8) (AA 1-60) (ABIN393490) Detection limit for recombinant GST tagged MED8 is approximately 0.1 ng/mL as a captu...
Background publications Léonard, Kohl, Hart, Elliott: "Interaction of Bunyamwera Orthobunyavirus NSs protein with mediator protein MED8: a mechanism for inhibiting the interferon response." in: Journal of virology, Vol. 80, Issue 19, pp. 9667-75, 2006

Wang, Yoon, Oh, Jeon, Kim, Kim, Byun, Yang, Kim, Kim, Yeom, Yoo, Kim, Kim: "Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags." in: Biochemical and biophysical research communications, Vol. 345, Issue 3, pp. 1022-32, 2006

Stelzl, Worm, Lalowski, Haenig, Brembeck, Goehler, Stroedicke, Zenkner, Schoenherr, Koeppen, Timm, Mintzlaff, Abraham, Bock, Kietzmann, Goedde, Toksöz, Droege, Krobitsch, Korn, Birchmeier, Lehrach et al.: "A human protein-protein interaction network: a resource for annotating the proteome. ..." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005

Zhang, Krutchinsky, Fukuda, Chen, Yamamura, Chait, Roeder: "MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription." in: Molecular cell, Vol. 19, Issue 1, pp. 89-100, 2005

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004