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Mediator Complex Subunit 8 (MED8) (AA 1-60) antibody

Details for Product No. ABIN393490
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Antigen
Synonyms zgc:76877, med8-a, ARC32, 2210021A15Rik, AB041805, MNCb-2386
Epitope
AA 1-60
(8), (8), (3), (2), (1), (1)
Reactivity
Human
(19), (5), (5), (1), (1)
Host
Mouse
(15), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(20), (14), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393490
450.00 $
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Immunogen MED8 (NP_443109, 1 a.a. ~ 60 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D3
Isotype IgG2a kappa
Specificity MED8 (NP_443109, 1 a.a. ~ 60 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MED8
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 29080 DA
Gene ID 12406
UniProt NP_443109
Research Area Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Mediator Complex Subunit 8 (MED8) (AA 1-60) antibody Detection limit for recombinant GST tagged MED8 is approximately 0.1 ng/mL as a capture antibody.
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Zhang, Krutchinsky, Fukuda et al.: "MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription." in: Molecular cell, Vol. 19, Issue 1, pp. 89-100, 2005 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome...." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Wang, Yoon, Oh et al.: "Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags." in: Biochemical and biophysical research communications, Vol. 345, Issue 3, pp. 1022-32, 2006 (PubMed).

Léonard, Kohl, Hart et al.: "Interaction of Bunyamwera Orthobunyavirus NSs protein with mediator protein MED8: a mechanism for inhibiting the interferon response." in: Journal of virology, Vol. 80, Issue 19, pp. 9667-75, 2006 (PubMed).

Validation Images
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