Mediator Complex Subunit 8 (MED8) antibody

Antigen: Mediator Complex Subunit 8 (MED8)

Synonyms: ARC32, MGC17544, MGC19641, med8

Clonality: Monoclonal (1D3)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393490

Additional Information

Alternative name: MED8

Gene ID: 112950

UniProt: NP_443109

Immunogen: MED8 (NP_443109, 1 a.a. ~ 60 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1D3

Description: Other names: ARC32, MGC17544, MGC19641mediator of RNA polymerase II transcription, subunit 8 homolog (yeast)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MED8 (NP_443109, 1 a.a. ~ 60 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 29080 DA

Comments:

Background: This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged MED8 is approximately 0.1ng/ml as a capture antibody.

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Zhang, Krutchinsky, Fukuda et al.: "MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription." in: Molecular cell, Vol. 19, Issue 1, pp. 89-100, 2005 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Wang, Yoon, Oh et al.: "Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags." in: Biochemical and biophysical research communications, Vol. 345, Issue 3, pp. 1022-32, 2006 (PubMed).

Léonard, Kohl, Hart et al.: "Interaction of Bunyamwera Orthobunyavirus NSs protein with mediator protein MED8: a mechanism for inhibiting the interferon response." in: Journal of virology, Vol. 80, Issue 19, pp. 9667-75, 2006 (PubMed).