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Details for Product No. ABIN393493

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody

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Antigen
Synonyms
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, pkb, xAct, akt-1, v-akt, v-akt1, Akt, AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AKT/PKB, Akt/PKB, Akt1, CG4006, D-Akt,  ... show more
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunofluorescence (IF), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393493
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen AKT1 (AAH00479, 1 a.a. ~ 481 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D4
Isotype IgG2a
Specificity AKT1 (AAH00479, 1 a.a. ~ 481 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKT1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 55686 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence
Comment

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Benesch, Schneider, Voelker et al.: "The clinicopathological and prognostic relevance of pyruvate kinase M2 and pAkt expression in breast cancer." in: Anticancer research, Vol. 30, Issue 5, pp. 1689-94, 2010 (PubMed).

Slattery, Herrick, Lundgreen et al.: "Genetic variation in a metabolic signaling pathway and colon and rectal cancer risk: mTOR, PTEN, STK11, RPKAA1, PRKAG2, TSC1, TSC2, PI3K and Akt1." in: Carcinogenesis, Vol. 31, Issue 9, pp. 1604-11, 2010 (PubMed).

Lin, Lin, Yu: "miR-149* induces apoptosis by inhibiting Akt1 and E2F1 in human cancer cells." in: Molecular carcinogenesis, Vol. 49, Issue 8, pp. 719-27, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Izquierdo, Truan, Mengual et al.: "HER-2/AKT expression in upper urinary tract urothelial carcinoma: prognostic implications." in: Anticancer research, Vol. 30, Issue 6, pp. 2439-45, 2010 (PubMed).

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts (655), (147), (8), (5), (4), (4)
Reactivities (795), (525), (502), (173), (154), (117), (95), (21), (19), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (590), (334), (205), (174), (143), (130), (116), (70), (31), (17), (17), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Conjugates (21), (15), (13), (13), (13), (13), (13), (13), (13), (13), (13), (9), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1)
Epitopes (123), (77), (45), (44), (43), (35), (30), (28), (27), (18), (16), (16), (9), (9), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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