S100 Calcium Binding Protein A1 (S100A1) (AA 1-76) antibody

Details for Product No. ABIN393494
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Antigen
Synonyms S100a1, S100A1, DKFZp468K0511, S100, S100-alpha, S100A, AI266795, S100a, NEF, S100-B, S100beta
Epitope
AA 1-76
(11), (8), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(141), (86), (52), (48), (12), (12), (11), (6), (2), (1)
Host
Mouse
(115), (77), (7), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (6), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(99), (93), (81), (55), (32), (24), (20), (10), (7), (6), (4), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393494
450.00 $
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Immunogen S100A1 (NP_006262, 1 a.a. ~ 76 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D5
Isotype IgG1 kappa
Specificity S100A1 (NP_006262, 1 a.a. ~ 76 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name S100A1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 10546 DA
Gene ID 12406
NCBI Accession NP_006262
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-76) antibody Western Blot analysis of S100A1 expression in transfected 293T cell line by S100A1 monoclonal antibody (M01), clone 1D5. Lane 1: S100A1 transfected lysate(10.5 KDa). Lane 2: Non-transfected lysate. Detection limit for recombinant GST tagged S100A1 is approximately 0.3 ng/mL as a capture antibody.
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-76) antibody (2) anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-76) antibody (Image 2)
Background publications Yusenko, Zubakov, Kovacs: "Gene expression profiling of chromophobe renal cell carcinomas and renal oncocytomas by Affymetrix GeneChip using pooled and individual tumours." in: International journal of biological sciences, Vol. 5, Issue 6, pp. 517-27, 2009 (PubMed).

DeRycke, Andersen, Harrington et al.: "S100A1 expression in ovarian and endometrial endometrioid carcinomas is a prognostic indicator of relapse-free survival." in: American journal of clinical pathology, Vol. 132, Issue 6, pp. 846-56, 2009 (PubMed).

Sviatoha, Tani, Kleina et al.: "Immunohistochemical analysis of the S100A1, S100B, CD44 and Bcl-2 antigens and the rate of cell proliferation assessed by Ki-67 antibody in benign and malignant melanocytic tumours." in: Melanoma research, Vol. 20, Issue 2, pp. 118-25, 2010 (PubMed).

Marlatt, Spratt, Shaw: "Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins." in: Protein expression and purification, Vol. 73, Issue 1, pp. 58-64, 2010 (PubMed).

van Dieck, Lum, Teufel et al.: "S100 proteins interact with the N-terminal domain of MDM2." in: FEBS letters, Vol. 584, Issue 15, pp. 3269-74, 2010 (PubMed).

Validation Images
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