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Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) (AA 117-226) antibody

Details for Product No. ABIN393496, Supplier: Log in to see
Antigen
  • PIMT
  • PCMT
  • C79501
  • PCM
Epitope
AA 117-226
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
42
22
19
1
1
1
1
1
1
Host
Mouse
36
6
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
30
13
11
8
6
5
4
1
1
1
1
1
Supplier
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Immunogen PCMT1 (NP_005380, 117 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D6
Isotype IgG1 kappa
Specificity PCMT1 (NP_005380, 117 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PCMT1 (PCMT1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 24650 DA
Gene ID 12406
NCBI Accession NP_005380
Research Area Signaling, Protein Modifications, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) (AA 117-226) antibody (ABIN393496) PCMT1 monoclonal antibody (M02), clone 1D6 Western Blot analysis of PCMT1 expression ...
Immunohistochemistry (IHC) image for anti-Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) (AA 117-226) antibody (ABIN393496) anti-Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) (AA 117-226) antibody (Image 2)
Background publications Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Martins-de-Souza, Maccarrone, Wobrock et al.: "Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 16, pp. 1176-89, 2010 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. ..." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Franke, Vermeulen, Steegers-Theunissen et al.: "An association study of 45 folate-related genes in spina bifida: Involvement of cubilin (CUBN) and tRNA aspartic acid methyltransferase 1 (TRDMT1)." in: Birth defects research. Part A, Clinical and molecular teratology, Vol. 85, Issue 3, pp. 216-26, 2009 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia." in: Journal of psychiatric research, Vol. 43, Issue 11, pp. 978-86, 2009 (PubMed).