Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) antibody

Antigen: Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1)

Clonality: Monoclonal (1D6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393496

Additional Information

Alternative name: PCMT1

Gene ID: 5110

UniProt: NP_005380

Immunogen: PCMT1 (NP_005380, 117 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1D6

Description: Other names: protein-L-isoaspartate (D-aspartate) O-methyltransferase

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: PCMT1 (NP_005380, 117 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 24650 DA

Comments:

Background: Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

PCMT1 monoclonal antibody (M02), clone 1D6 Western Blot analysis of PCMT1 expression in HeLa ( Cat # L013V1 ). Detection limit for recombinant GST tagged PCMT1 is approximately 0.1ng/ml as a capture antibody.

anti-Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1) antibody (Image 2)

Publications

Product: Martins-de-Souza, Gattaz, Schmitt et al.: "Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia." in: Journal of psychiatric research, Vol. 43, Issue 11, pp. 978-86, 2009 (PubMed).

Franke, Vermeulen, Steegers-Theunissen et al.: "An association study of 45 folate-related genes in spina bifida: Involvement of cubilin (CUBN) and tRNA aspartic acid methyltransferase 1 (TRDMT1)." in: Birth defects research. Part A, Clinical and molecular teratology, Vol. 85, Issue 3, pp. 216-26, 2009 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Martins-de-Souza, Maccarrone, Wobrock et al.: "Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 16, pp. 1176-89, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).