SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibody

Antigen: SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1)

Synonyms: KIAA1625, mKIAA1625, 4930431E10Rik, SMURF1, Smurf1

Clonality: Monoclonal (1D7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393498

Additional Information

Alternative name: SMURF1

Gene ID: 57154

UniProt: NP_065162

Immunogen: SMURF1 (NP_065162, 165 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1D7

Description: Other names: KIAA1625SMAD specific E3 ubiquitin protein ligase 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: SMURF1 (NP_065162, 165 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 86114 DA

Comments:

Background: This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Concentration: 0.5mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to SMURF1 (AT3966a) on HeLa cell . [antibody concentration 10 ug/ml] SMURF1 monoclonal antibody (M01), clone 1D7 Western Blot analysis of SMURF1 expression in HeLa ( Cat # L013V1 ).

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibody (Image 2)

Publications

Product: Crose, Hilder, Sciaky et al.: "Cerebral cavernous malformation 2 protein promotes smad ubiquitin regulatory factor 1-mediated RhoA degradation in endothelial cells." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13301-5, 2009 (PubMed).

Narimatsu, Bose, Pye et al.: "Regulation of planar cell polarity by Smurf ubiquitin ligases." in: Cell, Vol. 137, Issue 2, pp. 295-307, 2009 (PubMed).

Alarcón, Zaromytidou, Xi et al.: "Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-beta pathways." in: Cell, Vol. 139, Issue 4, pp. 757-69, 2009 (PubMed).

Li, Lu, Wang et al.: "Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation." in: Molecular and cellular biochemistry, Vol. 338, Issue 1-2, pp. 11-7, 2010 (PubMed).

Franke, Balschun, Sina et al.: "Genome-wide association study for ulcerative colitis identifies risk loci at 7q22 and 22q13 (IL17REL)." in: Nature genetics, Vol. 42, Issue 4, pp. 292-4, 2010 (PubMed).