SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibody

Details for Product No. ABIN393498
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Antigen
Synonyms mKIAA1625, 4930431E10Rik, RGD1309707
Reactivity
Human
(41), (26), (15), (13), (12), (12), (12)
Host
Mouse
(33), (6), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(30), (23), (16), (10), (5), (5), (3), (1), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN393498
Quantity 0.5 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen SMURF1 (NP_065162, 165 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D7
Isotype IgG2a kappa
Specificity SMURF1 (NP_065162, 165 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification Purified
Alternative Name SMURF1
Background This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined.
Synonyms: KIAA1625SMAD specific E3 ubiquitin protein ligase 1
Molecular Weight 86114 DA
Gene ID 57154
UniProt NP_065162
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined.

Restrictions For Research Use only
Concentration 0.5mg/ml
Buffer Phosphate buffered saline, pH 7.2
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibody Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell (antibody concentration 10 µg/mL). SMURF1 monoclonal antibody (M01), clone 1D7 Western Blot analysis of SMURF1 expression in HeLa.
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibody (2) anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibody (Image 2)
Background publications Crose, Hilder, Sciaky et al.: "Cerebral cavernous malformation 2 protein promotes smad ubiquitin regulatory factor 1-mediated RhoA degradation in endothelial cells." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13301-5, 2009 (PubMed).

Narimatsu, Bose, Pye et al.: "Regulation of planar cell polarity by Smurf ubiquitin ligases." in: Cell, Vol. 137, Issue 2, pp. 295-307, 2009 (PubMed).

Alarcón, Zaromytidou, Xi et al.: "Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-beta pathways." in: Cell, Vol. 139, Issue 4, pp. 757-69, 2009 (PubMed).

Li, Lu, Wang et al.: "Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation." in: Molecular and cellular biochemistry, Vol. 338, Issue 1-2, pp. 11-7, 2010 (PubMed).

Franke, Balschun, Sina et al.: "Genome-wide association study for ulcerative colitis identifies risk loci at 7q22 and 22q13 (IL17REL)." in: Nature genetics, Vol. 42, Issue 4, pp. 292-4, 2010 (PubMed).

Hosts (33), (6), (4)
Reactivities (41), (26), (15), (13), (12), (12), (12)
Applications (30), (23), (16), (10), (5), (5), (3), (1), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (4), (2), (2), (2), (2), (2), (1), (1)
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