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CBX5 antibody (Chromobox Homolog 5) (AA 1-192)

Details for Product anti-CBX5 Antibody No. ABIN393505, Supplier: Log in to see
Antigen
  • id:ibd2026
  • wu:fc63e02
  • wu:fe47g12
  • wu:fi03h05
  • zgc:158601
  • CBX5
  • cbx5
  • MGC64354
  • 2610029O15Rik
  • C75991
  • HP1
  • Hp1a
  • Hp1alpha
  • HP1A
Epitope
AA 1-192
23
17
13
13
13
13
8
7
7
6
6
5
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
142
69
30
7
4
2
1
1
Host
Mouse
125
21
19
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CBX5 antibody is un-conjugated
6
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
129
83
50
22
14
12
11
10
6
4
1
1
Supplier
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Immunogen CBX5 (AAH06821, 1 a.a. ~ 192 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E11-3A10
Isotype IgG1 kappa
Specificity CBX5 (AAH06821, 1 a.a. ~ 192 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CBX5 (CBX5 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 22225 DA
Gene ID 12406
Research Area Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-CBX5 antibody (Chromobox Homolog 5) (AA 1-192) (ABIN393505) Immunofluorescence of monoclonal antibody to CBX5 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-CBX5 antibody (Chromobox Homolog 5) (AA 1-192) (ABIN393505) anti-Chromobox Homolog 5 (CBX5) (AA 1-192) antibody (Image 2)
Background publications Nozawa, Nagao, Masuda et al.: "Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation." in: Nature cell biology, Vol. 12, Issue 7, pp. 719-27, 2010 (PubMed).

Chaturvedi, Parnaik: "Lamin A rod domain mutants target heterochromatin protein 1alpha and beta for proteasomal degradation by activation of F-box protein, FBXW10." in: PLoS ONE, Vol. 5, Issue 5, pp. e10620, 2010 (PubMed).

Kiyomitsu, Iwasaki, Obuse et al.: "Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes." in: The Journal of cell biology, Vol. 188, Issue 6, pp. 791-807, 2010 (PubMed).

Emelyanov, Konev, Vershilova et al.: "Protein complex of Drosophila ATRX/XNP and HP1a is required for the formation of pericentric beta-heterochromatin in vivo." in: The Journal of biological chemistry, Vol. 285, Issue 20, pp. 15027-37, 2010 (PubMed).

Wong, McGhie, Sim et al.: "ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells." in: Genome research, Vol. 20, Issue 3, pp. 351-60, 2010 (PubMed).