Chromobox Homolog 5 (CBX5) antibody

Antigen: Chromobox Homolog 5 (CBX5)

Synonyms: MGC158601, id:ibd2026, wu:fc63e02, wu:fe47g12, wu:fi03h05, zgc:158601, CBX5, cbx5, MGC64354, MGC76213, MGC89578, HP1, HP1A, Hp1a, C75991, 2610029O15Rik

Clonality: Monoclonal (1E11-3A10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393505

Additional Information

Alternative name: CBX5

Gene ID: 23468

Immunogen: CBX5 (AAH06821, 1 a.a. ~ 192 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1E11-3A10

Description: Other names: HP1, HP1ALPHA, HP1Hsalpha chromobox homolog 5 (HP1 alpha homolog, Drosophila)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CBX5 (AAH06821, 1 a.a. ~ 192 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 22225 DA

Comments:

Background: This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to CBX5 (AT1411a) on HeLa cell. [antibody concentration 10ug/ml] CBX5 monoclonal antibody (M01), clone 1E11-3A10 Western Blot analysis of CBX5 expression in HeLa NE ( Cat # L013V3 ).

anti-Chromobox Homolog 5 (CBX5) antibody (Image 2)

Publications

Product: Wong, McGhie, Sim et al.: "ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells." in: Genome research, Vol. 20, Issue 3, pp. 351-60, 2010 (PubMed).

Emelyanov, Konev, Vershilova et al.: "Protein complex of Drosophila ATRX/XNP and HP1a is required for the formation of pericentric beta-heterochromatin in vivo." in: The Journal of biological chemistry, Vol. 285, Issue 20, pp. 15027-37, 2010 (PubMed).

Kiyomitsu, Iwasaki, Obuse et al.: "Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes." in: The Journal of cell biology, Vol. 188, Issue 6, pp. 791-807, 2010 (PubMed).

Chaturvedi, Parnaik: "Lamin A rod domain mutants target heterochromatin protein 1alpha and beta for proteasomal degradation by activation of F-box protein, FBXW10." in: PLoS ONE, Vol. 5, Issue 5, pp. e10620, 2010 (PubMed).

Nozawa, Nagao, Masuda et al.: "Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation." in: Nature cell biology, Vol. 12, Issue 7, pp. 719-27, 2010 (PubMed).