Chromobox Homolog 5 (CBX5) antibody

Details for Product No. ABIN393505
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Antigen
Synonyms HP1, HP1A, 2610029O15Rik, C75991, Hp1a, Hp1alpha, id:ibd2026, wu:fc63e02, wu:fe47g12, wu:fi03h05, zgc:158601, CBX5, cbx5, MGC64354
Reactivity
Human
(106), (52), (34), (30), (25), (2), (1)
Host
Mouse
(95), (13), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(78), (49), (30), (29), (17), (8), (6), (4), (3)
Pubmed 5 references available
Catalog no. ABIN393505
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen CBX5 (AAH06821, 1 a.a. ~ 192 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E11-3A10
Isotype IgG1 kappa
Specificity CBX5 (AAH06821, 1 a.a. ~ 192 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CBX5
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 22225 DA
Gene ID 12406
Research Area Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Chromobox Homolog 5 (CBX5) antibody Immunofluorescence of monoclonal antibody to CBX5 on HeLa cell (antibody concentration 10 µg/mL). CBX5 monoclonal antibody (M01), clone 1E11-3A10 Western Blot analysis of CBX5 expression in HeLa NE.
anti-Chromobox Homolog 5 (CBX5) antibody (2) anti-Chromobox Homolog 5 (CBX5) antibody (Image 2)
Background publications Wong, McGhie, Sim et al.: "ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells." in: Genome research, Vol. 20, Issue 3, pp. 351-60, 2010 (PubMed).

Emelyanov, Konev, Vershilova et al.: "Protein complex of Drosophila ATRX/XNP and HP1a is required for the formation of pericentric beta-heterochromatin in vivo." in: The Journal of biological chemistry, Vol. 285, Issue 20, pp. 15027-37, 2010 (PubMed).

Kiyomitsu, Iwasaki, Obuse et al.: "Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes." in: The Journal of cell biology, Vol. 188, Issue 6, pp. 791-807, 2010 (PubMed).

Chaturvedi, Parnaik: "Lamin A rod domain mutants target heterochromatin protein 1alpha and beta for proteasomal degradation by activation of F-box protein, FBXW10." in: PLoS ONE, Vol. 5, Issue 5, pp. e10620, 2010 (PubMed).

Nozawa, Nagao, Masuda et al.: "Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation." in: Nature cell biology, Vol. 12, Issue 7, pp. 719-27, 2010 (PubMed).

Hosts (95), (13), (7)
Reactivities (106), (52), (34), (30), (25), (2), (1)
Applications (78), (49), (30), (29), (17), (8), (6), (4), (3)
Conjugates (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Epitopes (15), (12), (12), (8), (7), (7), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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