Ephrin A5 (EFNA5) antibody

Antigen: Ephrin A5 (EFNA5)

Synonyms: AF1, EFL5, RAGS, EPLG7, GLC1M, LERK7, EFNA5, af1, efl5, rags, eplg7, lerk7, AL-1, Epl7, EFL-5, LERK-7, AV158822, Ephrin-A5, Lerk7

Clonality: Monoclonal (1F12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393511

Additional Information

Alternative name: EFNA5

Gene ID: 1946

UniProt: NP_001953

Immunogen: EFNA5 (NP_001953, 114 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1F12

Description: Other names: AF1, EFL5, EPLG7, LERK7, RAGS ephrin-A5

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: EFNA5 (NP_001953, 114 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 26297 DA

Comments:

Background: Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

EFNA5 monoclonal antibody (M01), clone 1F12 Western Blot analysis of EFNA5 expression in NIH/3T3 ( Cat # L018V1 ). Detection limit for recombinant GST tagged EFNA5 is approximately 0.03ng/ml as a capture antibody.

anti-Ephrin A5 (EFNA5) antibody (Image 2)

Publications

Product: Potkin, Guffanti, Lakatos et al.: "Hippocampal atrophy as a quantitative trait in a genome-wide association study identifying novel susceptibility genes for Alzheimer's disease." in: PLoS ONE, Vol. 4, Issue 8, pp. e6501, 2009 (PubMed).

Kalinski, Röpke, Sel et al.: "Down-regulation of ephrin-A5, a gene product of normal cartilage, in chondrosarcoma." in: Human pathology, Vol. 40, Issue 12, pp. 1679-85, 2009 (PubMed).

Sokolowski, Wasserman, Wasserman: "Association of polymorphisms in the SLIT2 axonal guidance gene with anger in suicide attempters." in: Molecular psychiatry, Vol. 15, Issue 1, pp. 10-1, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Kariuki, Franek, Kumar et al.: "Trait-stratified genome-wide association study identifies novel and diverse genetic associations with serologic and cytokine phenotypes in systemic lupus erythematosus." in: Arthritis research & therapy, Vol. 12, Issue 4, pp. R151, 2010 (PubMed).