|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Defender Against Cell Death 1 (DAD1) antibody
|Synonyms||OST2, AI323713, dad1, NV18456, DAD1, DKFZp469O2339, ost2, DAD-1, si:ch211-9d9.5|
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||DAD1 (AAH07403, 1 a.a. ~ 114 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: OST2 defender against cell death 1|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||DAD1 (AAH07403, 1 a.a. ~ 114 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||12497 DA|
Background: DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged DAD1 is approximately 0.3ng/ml as a capture antibody.|
Shibatani, David, McCormack et al.: "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." in: Biochemistry, Vol. 44, Issue 16, pp. 5982-92, 2005 (PubMed).
Kulke, Freed, Chiang et al.: "High-resolution analysis of genetic alterations in small bowel carcinoid tumors reveals areas of recurrent amplification and loss." in: Genes, chromosomes & cancer, Vol. 47, Issue 7, pp. 591-603, 2008 (PubMed).
Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).
Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).
Shimada, Miyagawa, Kawashima et al.: "An approach based on a genome-wide association study reveals candidate loci for narcolepsy." in: Human genetics, Vol. 128, Issue 4, pp. 433-41, 2010 (PubMed).
|Reactivities||Human (38), Mouse (Murine) (30), Rat (Rattus) (21), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (23), Immunofluorescence (IF) (19), ELISA (15), Immunocytochemistry (ICC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Biotin (2), FITC (2), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (5), C-Term,AA 63-92 (1)|