Defender Against Cell Death 1 (DAD1) antibody

Antigen: Defender Against Cell Death 1 (DAD1)

Synonyms: OST2, AI323713, dad1, NV18456, DAD1, DKFZp469O2339, ost2, DAD-1, si:ch211-9d9.5

Clonality: Monoclonal (1F3-A4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN393515

Additional Information

Alternative name: DAD1

Gene ID: 1603

Immunogen: DAD1 (AAH07403, 1 a.a. ~ 114 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 1F3-A4

Description: Other names: OST2 defender against cell death 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DAD1 (AAH07403, 1 a.a. ~ 114 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 12497 DA

Comments:

Background: DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged DAD1 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Shibatani, David, McCormack et al.: "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." in: Biochemistry, Vol. 44, Issue 16, pp. 5982-92, 2005 (PubMed).

Kulke, Freed, Chiang et al.: "High-resolution analysis of genetic alterations in small bowel carcinoid tumors reveals areas of recurrent amplification and loss." in: Genes, chromosomes & cancer, Vol. 47, Issue 7, pp. 591-603, 2008 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Shimada, Miyagawa, Kawashima et al.: "An approach based on a genome-wide association study reveals candidate loci for narcolepsy." in: Human genetics, Vol. 128, Issue 4, pp. 433-41, 2010 (PubMed).