E2F Transcription Factor 6 (E2F6) antibody

Antigen: E2F Transcription Factor 6 (E2F6)

Synonyms: E2F-6, MGC111545, EMA, E2F6b, AI462434, E2F-4, AI427446, 2010111M04Rik

Clonality: Monoclonal (1F7-A9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393521

Additional Information

Alternative name: E2F6

Gene ID: 1876

Immunogen: E2F6 (AAH08348, 1 a.a. ~ 282 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1F7-A9

Description: Other names: E2F6E2F transcription factor 6

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: E2F6 (AAH08348, 1 a.a. ~ 282 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 31844 DA

Comments:

Background: This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to E2F6 (AT1838a) on HeLa cell . [antibody concentration 10 ug/ml] E2F6 monoclonal antibody (M02), clone 1F7-A9. Western Blot analysis of E2F6 expression in HepG2 ( Cat # L019V1 ).

anti-E2F Transcription Factor 6 (E2F6) antibody (Image 2)

Publications

Product: Tahiliani, Mei, Fang et al.: "The histone H3K4 demethylase SMCX links REST target genes to X-linked mental retardation." in: Nature, Vol. 447, Issue 7144, pp. 601-5, 2007 (PubMed).

Kikuchi, Shimizu, Wada et al.: "E2F-6 suppresses growth-associated apoptosis of human hematopoietic progenitor cells by counteracting proapoptotic activity of E2F-1." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 10, pp. 2439-47, 2007 (PubMed).

Xu, Bieda, Jin et al.: "A comprehensive ChIP-chip analysis of E2F1, E2F4, and E2F6 in normal and tumor cells reveals interchangeable roles of E2F family members." in: Genome research, Vol. 17, Issue 11, pp. 1550-61, 2007 (PubMed).

Yang, Shu, Zhu et al.: "E2F6 inhibits cobalt chloride-mimetic hypoxia-induced apoptosis through E2F1." in: Molecular biology of the cell, Vol. 19, Issue 9, pp. 3691-700, 2008 (PubMed).

Cunningham, Vierkant, Sellers et al.: "Cell cycle genes and ovarian cancer susceptibility: a tagSNP analysis." in: British journal of cancer, Vol. 101, Issue 8, pp. 1461-8, 2009 (PubMed).